Tested Applications
| Positive WB detected in | A549 cells, L02 cells, HepG2 cells, mouse liver tissue, mouse kidney tissue, MCF-7 cells, mouse pancreas tissue, rat pancreas tissue, A375 cells |
| Positive IHC detected in | human thyroid cancer tissue, human breast cancer tissue, human lung cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11521-1-AP targets ECM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2084 Product name: Recombinant human ECM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 241-540 aa of BC023505 Sequence: MSRFCEAEFSVKTRPHWCCTRQGEARFSCFQEEAPQPHYQLRACPSHQPDISSGLELPFPPGVPTLDNIKNICHLRRFRSVPRNLPATDPLQRELLALIQLEREFQRCCRQGNNHTCTWKAWEDTLDKYCDREYAVKTHHHLCCRHPPSPTRDECFARRAPYPNYDRDILTIDISRVTPNLMGHLCGNQRVLTKHKHIPGLIHNMTARCCDLPFPEQACCAEEEKLTFINDLCGPRRNIWRDPALCCYLSPGDEQVNCFNINYLRNVALVSGDTENAKGQGEQGSTGGTNISSTSEPKEE Predict reactive species |
| Full Name | extracellular matrix protein 1 |
| Calculated Molecular Weight | 540 aa, 61 kDa |
| Observed Molecular Weight | 55-61 kDa |
| GenBank Accession Number | BC023505 |
| Gene Symbol | ECM1 |
| Gene ID (NCBI) | 1893 |
| RRID | AB_2261964 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16610 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis.ECM1 is expressed in breast cancer and could participate in cell migration and angiogenesis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers.(PMID:21128013) It inhibits MMP9 proteolytic activity. ECM1 protein is a possible trigger for angiogenesis, tumor progression and malignancies(PMID:21497598).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ECM1 antibody 11521-1-AP | Download protocol |
| IHC protocol for ECM1 antibody 11521-1-AP | Download protocol |
| WB protocol for ECM1 antibody 11521-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-ECM1 antibody (Cat# 11521-1-AP) has 35 citations across journals including Nature Metabolism, Advanced Science, Cancer Letters, Oncogene, Cell Reports, Free Radical Biology and Medicine, and Journal of Biological Chemistry. Research fields span oncology (prostate, breast, liver, gastric, pancreatic, colorectal cancers), fibrosis, nephrology, dermatology, reproductive biology, neuroscience, and proteomics. Applications include WB, IHC, IF, and IP in human, mouse, and canine samples.
| Species | Application | Title |
|---|---|---|
Nat Metab Early-activated extracellular matrix proteins shape the metabolic and spatial dynamics of the kidney fibrotic microenvironment.
| ||
Adv Sci (Weinh) Osteoblast-Derived ECM1 Promotes Anti-Androgen Resistance in Bone Metastatic Prostate Cancer | ||
Cancer Lett Liver metastasis or peritoneal metastasis: single-cell RNA sequencing reveals the organotropism in colorectal cancer is driven by distinct partial-EMT processes | ||
Int J Biol Sci Mechanistic study of celastrol-mediated inhibition of proinflammatory activation of macrophages in IgA nephropathy via down-regulating ECM1 | ||
Cell Death Discov ECM1 modified HF-MSCs targeting HSC attenuate liver cirrhosis by inhibiting the TGF-β/Smad signaling pathway. | ||
Acta Pharmacol Sin Nobiletin and polydatin synergistically alleviate triple-negative breast cancer liver metastasis via suppressing ECM1a-mediated fatty acid biosynthesis. |
