ECM1 Polyclonal antibody

ECM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

25

Cat no : 11521-1-AP

Synonyms

Secretory component p85, extracellular matrix protein 1

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Validation Data Gallery

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  WB analysis of A549 using 11521-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11521-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11521-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 11521-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11521-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11521-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11521-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11521-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11521-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human thyroid cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11521-1-AP (ECM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11521-1-AP (ECM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 25x lens).

• 

  IHC staining of human thyroid cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thyroid cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 11521-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 25x lens).

• 

  IF Staining of HepG2 using 11521-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11521-1-AP (ECM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11521-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ECM1 antibody (11521-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, L02 cells, HepG2 cells, mouse liver tissue, mouse kidney tissue, MCF-7 cells, mouse pancreas tissue, rat pancreas tissue, A375 cells
• Positive IHC detected in: human thyroid cancer tissue, human breast cancer tissue, human lung cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IHC: See 11 publications below
• IF: See 5 publications below

Product Information

11521-1-AP targets ECM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ECM1 fusion protein Ag2084
• Full Name: extracellular matrix protein 1
• Calculated Molecular Weight: 540 aa, 61 kDa
• Observed Molecular Weight: 55-61 kDa
• GenBank Accession Number: BC023505
• Gene Symbol: ECM1
• Gene ID (NCBI): 1893
• RRID: AB_2261964
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis.ECM1 is expressed in breast cancer and could participate in cell migration and angiogenesis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers.(PMID:21128013) It inhibits MMP9 proteolytic activity. ECM1 protein is a possible trigger for angiogenesis, tumor progression and malignancies(PMID:21497598).

Publications

Species	Application	Title
		Dev Cell; Spatial Control of Primary Ciliogenesis by Subdistal Appendages Alters Sensation-Associated Properties of Cilia.; Authors - Gregory Mazo
human	IF	Oncogene; Extracellular matrix protein 1 promotes cell metastasis and glucose metabolism by inducing integrin β4/FAK/SOX2/HIF-1α signaling pathway in gastric cancer.; Authors - L Gan
human	WB,IHC,IF	J Biol Chem; Phospholipid scramblase 1 is secreted by a lipid raft-dependent pathway and interacts with the extracellular matrix protein 1 in the dermal epidermal junction zone of human skin.; Authors - Merregaert Joseph J
human	WB,IHC	Am J Transl Res; miR-23a-5p inhibits cell proliferation and invasion in pancreatic ductal adenocarcinoma by suppressing ECM1 expression.; Authors - Wenjie Huang
human	IHC	Food Chem Toxicol; MiR-92a antagonized the facilitation effect of extracellular matrix protein 1 in GC metastasis through targeting its 3'UTR region.; Authors - Lin-Qing Liu
human	IHC,IF	Matrix Biol; ECM1 interacts with fibulin-3 and the beta 3 chain of laminin 332 through its serum albumin subdomain-like 2 domain.; Authors - Sercu S S