ChromoTek EGFP, recombinant purified protein

Validation Data Gallery

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  SDS-PAGE of EGFP: 10 % acrylamide gel; 1 µg of EGFP has been loaded. Detection: InstantBlue

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  Normalized EGFP fluorescence excitation and emission spectra.

Purified recombinant Enhanced Green Fluorescent Protein (EGFP); full length protein; fused to 6xHis-tag.

Cat No. EGFP

Publications(11)

Protein

EGFP

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Synonyms

EGFP

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Product Information

Purified recombinant Enhanced Green Fluorescent Protein (EGFP); full length protein; fused to 6xHis-tag.

• Applications: Immunoprecipitation: use ~ 5 μg EGFP per 25 μL GFP-Trap® bead slurry; SDS-PAGE: use ~ 1 μg EGFP per lane; Western blot: use ~ 25 ng - 100 ng EGFP per lane; ELISA: coat ~ 1ng - 50 ng per well (96-well plate); Other in vitro assays: as a fluorescent standard and for calibration
• Molecular Weight: 28.0 kDa (including the polyhistidine-tag)
• Form: Liquid
• Size: 250 µL
• Concentration: 1 g/L
• Conjugate: Unconjugated
• Purity: > 80% by SDS-PAGE
• Source: Escherichia coli
• Storage Buffer: 0.09% sodium azide
• Storage Condition: Shipped at +4°C. ; Store at +4°C short term (up to 1 week). ; Store at -20°C or -80°C long term. ; Avoid freeze-thaw cycles. Stable for one year.

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Publications

Application	Title
	Cell; Cellular Control of Viscosity Counters Changes in Temperature and Energy Availability.; Authors - Laura B Persson
	Microsyst Nanoeng; Employing electrochemically derived pH gradients for Lab-on-PCB protein preconcentration devices; Authors - Grace Maxted
IP	PLoS Pathog; New insights into the role of endosomal proteins for African swine fever virus infection.; Authors - Miguel Ángel Cuesta-Geijo
WB	Int J Mol Sci; Comparison of Multiple Strategies for Precision Transgene Knock-In in Gallus gallus Genome via Microhomology-Mediated End Joining; Authors - Lijuan Wang
	bioRxiv; Adhesion energy controls lipid binding-mediated endocytosis; Authors - Raluca Groza
	Int J Mol Sci; The Thermodynamic Fingerprints of Ultra-Tight Nanobody-Antigen Binding Probed via Two-Color Single-Molecule Coincidence Detection; Authors - Benno Schedler