EI24 Polyclonal antibody
EI24 Polyclonal Antibody for WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB,ELISA
Conjugate
Unconjugated
Cat no : 20456-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, HT-1080 cells, HeLa cells, L02 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 20456-1-AP is EI24 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | EI24 fusion protein Ag14169 |
Full Name | etoposide induced 2.4 mRNA |
Calculated molecular weight | 340 aa, 39 kDa |
Observed molecular weight | 40-45 kDa |
GenBank accession number | BC002390 |
Gene symbol | EI24 |
Gene ID (NCBI) | 9538 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ei24 is an ER-localized protein originally identified as a p53-induced proapoptotic gene in etoposide-treated NIH3T3 cells. It has been shown to be able to bind to the antiapoptotic protein Bcl-2 (PMID: 15781622), play a role in autophagy (PMID: 23074225), and induce proliferate arrest/apoptosis (PMID: 10594026). Ei24 can bind specifically to IMPβ1 and IMPα2 to impede their normal role in nuclear import (PMID: 24821838).
Protocols
Product Specific Protocols | |
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WB protocol for EI24 antibody 20456-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |