EI24 Polyclonal antibody

EI24 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20456-1-AP

Synonyms

EI24, etoposide induced 2.4 mRNA, p53 induced gene 8 protein, p53-induced factor Ei24, PIG8, TP53I8



Tested Applications

Positive WB detected inHeLa cells, HT-1080 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20456-1-AP is EI24 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EI24 fusion protein Ag14169
Full Name etoposide induced 2.4 mRNA
Calculated molecular weight 340 aa, 39 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC002390
Gene symbol EI24
Gene ID (NCBI) 9538
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ei24 is an ER-localized protein originally identified as a p53-induced proapoptotic gene in etoposide-treated NIH3T3 cells. It has been shown to be able to bind to the antiapoptotic protein Bcl-2 (PMID: 15781622), play a role in autophagy (PMID: 23074225), and induce proliferate arrest/apoptosis (PMID: 10594026). Ei24 can bind specifically to IMPβ1 and IMPα2 to impede their normal role in nuclear import (PMID: 24821838).

Protocols

Product Specific Protocols
WB protocol for EI24 antibody 20456-1-APDownload protocol
Standard Protocols
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