PERK/EIF2AK3 Polyclonal antibody

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  WB analysis using 24390-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24390-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 24390-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 24390-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PERK/EIF2AK3 (24390-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

PERK/EIF2AK3 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 24390-1-AP

Publications(172)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (8)

Applications

WB, IHC, IF, FC (Intra), IP, CoIP, ELISA

Synonyms

EIF2AK3, PERK, EC:2.7.11.1, EIF2AK 3, Eukaryotic translation initiation factor 2-alpha kinase 3

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 160 publications below
• IHC: See 7 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

24390-1-AP targets PERK/EIF2AK3 in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, monkey, chicken, bovine, hamster, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18254

  Product name: Recombinant human EIF2AK3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 767-1116 aa of BC126354

  Sequence: TDCDVEDGTMDGNDEGHSFELCPSEASPYVRSRERTSSSIVFEDSGCDNASSKEEPKTNRLHIGNHCANKLTAFKPTSSKSSSEATLSISPPRPTTLSLDLTKNTTEKLQPSSPKVYLYIQMQLCRKENLKDWMNGRCTIEERERSVCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQTVLTPMPAYARHTGQVGTKLYMSPEQIHGNSYSHKVDIFSLGLILFELLYPFSTQMERVRTLTDVRNLKFPPLFTQKYPCEYVMVQDMLSPSPMERPEAINIIENAVFEDLDFPGKTVLRQRSRSLSSSGTKHSRQSNNSHSPLPSN

• Full Name: eukaryotic translation initiation factor 2-alpha kinase 3
• Calculated Molecular Weight: 1116 aa, 125 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC126354
• Gene Symbol: PERK
• Gene ID (NCBI): 9451
• RRID: AB_2879521
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NZJ5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Nature; Ageing promotes metastasis via activation of the integrated stress response; Authors - Angana A H Patel
mouse	WB	Nat Commun; Activation of GCN2/ATF4 signals in amygdalar PKC-δ neurons promotes WAT browning under leucine deprivation.; Authors - Feixiang Yuan
		J Adv Res; Postantibiotic leukocyte enhancement-mediated reduction of intracellular bacteria by macrophages; Authors - Yifan Wu
pig	WB	Autophagy; Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy.; Authors - Sheng-Li Ming
human	WB	Autophagy; Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.; Authors - Tengfei Huang
pig	WB	Autophagy; STING1 is essential for an RNA-virus triggered autophagy.; Authors - Rui Zhang ; KD Validated