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ENO1 Polyclonal antibody

ENO1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 11204-1-AP


Alpha enolase, C myc promoter binding protein, ENO1, ENO1L1, Enolase 1, enolase 1, (alpha), MBP 1, MBPB1, MPB 1, MPB1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH

Tested Applications

Positive WB detected inHeLa cells, human skeletal muscle tissue, SGC-7901 cells, mouse skeletal muscle tissue, PC-3 cells, L02 cells, mouse liver tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11204-1-AP targets ENO1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENO1 fusion protein Ag1692
Full Name enolase 1, (alpha)
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC015641
Gene symbol ENO1
Gene ID (NCBI) 2023
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate.Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells .And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation.(19756395)


Product Specific Protocols
WB protocol for ENO1 antibody 11204-1-APDownload protocol
IHC protocol for ENO1 antibody 11204-1-APDownload protocol
IF protocol for ENO1 antibody 11204-1-APDownload protocol
IP protocol for ENO1 antibody 11204-1-APDownload protocol
FC protocol for ENO1 antibody 11204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.

Authors - Erika C Urdaneta


PMID: 31480692

Authors - Xixi Guo

J Mol Histol

Gamma-enolase predicts lung damage in severe acute pancreatitis-induced acute lung injury.

Authors - Lawrence Owusu

Nat Metab

Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.

Authors - Shi-Ting Li

Cell Rep

Enhancer Reprogramming Confers Dependence on Glycolysis and IGF Signaling in KMT2D Mutant Melanoma.

Authors - Mayinuer Maitituoheti

Cell Death Dis

POU6F1 cooperates with RORA to suppress the proliferation of lung adenocarcinoma by downregulation HIF1A signaling pathway.

Authors - Wenjing Xiao


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Kihsu (Verified Customer) (06-10-2021)

its gave good looking results in WB

  • Applications: Western Blot
  • Cell Tissue Type: mouse liver tissues

Kristian (Verified Customer) (06-07-2021)

Enolase 1 (green) is detected in the proximal tubules where HIF1A-signaling is activated.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Transgenic mouse kidney (TRACK)
ENO1 Antibody Immunofluorescence validation (1:200 dilution) in Transgenic mouse kidney (TRACK) (Cat no:11204-1-AP)