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ENO1 Polyclonal antibody
ENO1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 11204-1-AP
Validation Data Gallery
|Positive WB detected in
|HeLa cells, human skeletal muscle tissue, mouse skeletal muscle tissue, L02 cells, PC-3 cells, SGC-7901 cells, mouse liver tissue
|Positive IP detected in
|mouse skeletal muscle tissue
|Positive IHC detected in
|human stomach cancer tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:12000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:500-1:2000
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11204-1-AP targets ENO1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|ENO1 fusion protein Ag1692
|enolase 1, (alpha)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells. And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation. (PMID: 19756395)
|Product Specific Protocols
|WB protocol for ENO1 antibody 11204-1-AP
|IHC protocol for ENO1 antibody 11204-1-AP
|IF protocol for ENO1 antibody 11204-1-AP
|IP protocol for ENO1 antibody 11204-1-AP
|Click here to view our Standard Protocols
Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.
The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.
Riboregulation of Enolase 1 activity controls glycolysis and embryonic stem cell differentiation.
Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.
Nucleic Acids Res
FAX-RIC enables robust profiling of dynamic RNP complex formation in multicellular organisms in vivo.
Clin Transl Med
METTL3-mediated N6-methyladenosine exacerbates ferroptosis via m6A-IGF2BP2-dependent mitochondrial metabolic reprogramming in sepsis-induced acute lung injury
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kihsu (Verified Customer) (06-10-2021)
its gave good looking results in WB
Kristian (Verified Customer) (06-07-2021)
Enolase 1 (green) is detected in the proximal tubules where HIF1A-signaling is activated.