|Positive WB detected in||HeLa cells, human skeletal muscle tissue, SGC-7901 cells, mouse skeletal muscle tissue, PC-3 cells, L02 cells, mouse liver tissue|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||human colon cancer tissue, human liver cancer tissue, human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11204-1-AP targets ENO1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ENO1 fusion protein Ag1692|
|Full Name||enolase 1, (alpha)|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC015641|
|Gene ID (NCBI)||2023|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate.Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells .And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation.(19756395)
|Product Specific Protocols|
|WB protocol for ENO1 antibody 11204-1-AP||Download protocol|
|IHC protocol for ENO1 antibody 11204-1-AP||Download protocol|
|IF protocol for ENO1 antibody 11204-1-AP||Download protocol|
|IP protocol for ENO1 antibody 11204-1-AP||Download protocol|
|FC protocol for ENO1 antibody 11204-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.
J Mol Histol
Gamma-enolase predicts lung damage in severe acute pancreatitis-induced acute lung injury.
Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.
Enhancer Reprogramming Confers Dependence on Glycolysis and IGF Signaling in KMT2D Mutant Melanoma.
Can J Physiol Pharmacol
Upregulation of α-enolase protects cardiomyocytes from phenylephrine-induced hypertrophy.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kihsu (Verified Customer) (06-10-2021)
its gave good looking results in WB
Kristian (Verified Customer) (06-07-2021)
Enolase 1 (green) is detected in the proximal tubules where HIF1A-signaling is activated.