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- KD/KO Validated
ENO1 Polyclonal antibody
ENO1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse and More (1)
WB, IP, IHC, IF, FC, CoIP, ELISA
Cat no : 11204-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, human skeletal muscle tissue, mouse skeletal muscle tissue, L02 cells, PC-3 cells, SGC-7901 cells, mouse liver tissue|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||human stomach cancer tissue, human colon cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||A549 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:2000-1:16000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 8 publications below|
|WB||See 48 publications below|
|IHC||See 12 publications below|
|IF||See 5 publications below|
|IP||See 4 publications below|
|CoIP||See 1 publications below|
11204-1-AP targets ENO1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ENO1 fusion protein Ag1692|
|Full Name||enolase 1, (alpha)|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC015641|
|Gene ID (NCBI)||2023|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells. And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation. (PMID: 19756395)
|Product Specific Protocols|
|WB protocol for ENO1 antibody 11204-1-AP||Download protocol|
|IHC protocol for ENO1 antibody 11204-1-AP||Download protocol|
|IF protocol for ENO1 antibody 11204-1-AP||Download protocol|
|IP protocol for ENO1 antibody 11204-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.
The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.
Riboregulation of Enolase 1 activity controls glycolysis and embryonic stem cell differentiation.
Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.
Nucleic Acids Res
FAX-RIC enables robust profiling of dynamic RNP complex formation in multicellular organisms in vivo.
cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kihsu (Verified Customer) (06-10-2021)
its gave good looking results in WB
Kristian (Verified Customer) (06-07-2021)
Enolase 1 (green) is detected in the proximal tubules where HIF1A-signaling is activated.