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  • KD/KO Validated

ENO1 Polyclonal antibody

ENO1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11204-1-AP

Synonyms

Alpha enolase, C myc promoter binding protein, ENO1, ENO1L1, Enolase 1, enolase 1, (alpha), MBP 1, MBPB1, MPB 1, MPB1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH



Tested Applications

Positive WB detected inHeLa cells, human skeletal muscle tissue, mouse skeletal muscle tissue, L02 cells, PC-3 cells, SGC-7901 cells, mouse liver tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman stomach cancer tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11204-1-AP targets ENO1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENO1 fusion protein Ag1692
Full Name enolase 1, (alpha)
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC015641
Gene symbol ENO1
Gene ID (NCBI) 2023
RRIDAB_2099064
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells. And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation. (PMID: 19756395)

Protocols

Product Specific Protocols
WB protocol for ENO1 antibody 11204-1-APDownload protocol
IHC protocol for ENO1 antibody 11204-1-APDownload protocol
IF protocol for ENO1 antibody 11204-1-APDownload protocol
IP protocol for ENO1 antibody 11204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Neurosci

Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.

Authors - Xiaoxiang Dong
humanWB,IF,IP

Mol Cell

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Authors - Youming Zhu
mouseWB,IP

Mol Cell

Riboregulation of Enolase 1 activity controls glycolysis and embryonic stem cell differentiation.

Authors - Ina Huppertz
humanWB

Nat Commun

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Authors - Xiao-Hui Wang
humanWB

Nucleic Acids Res

FAX-RIC enables robust profiling of dynamic RNP complex formation in multicellular organisms in vivo.

Authors - Yongwoo Na
mouseWB

Clin Transl Med

METTL3-mediated N6-methyladenosine exacerbates ferroptosis via m6A-IGF2BP2-dependent mitochondrial metabolic reprogramming in sepsis-induced acute lung injury

Authors - Hao Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kihsu (Verified Customer) (06-10-2021)

its gave good looking results in WB

  • Applications: Western Blot
  • Cell Tissue Type: mouse liver tissues
FH

Kristian (Verified Customer) (06-07-2021)

Enolase 1 (green) is detected in the proximal tubules where HIF1A-signaling is activated.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Transgenic mouse kidney (TRACK)
ENO1 Antibody Immunofluorescence validation (1:200 dilution) in Transgenic mouse kidney (TRACK) (Cat no:11204-1-AP)