Cardinal Red™-conjugated ENO1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ENO1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

Cardinal Red™ Fluorescent Dye

Cat no : CR-11204

Synonyms

Alpha enolase, C myc promoter binding protein, ENO1, ENO1L1, Enolase 1, enolase 1, (alpha), MBP 1, MBPB1, MPB 1, MPB1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH

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Validation Data Gallery

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  FC experiment of HeLa using CR-11204

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Cardinal Red™ Anti-Human ENO1 (CR-11204) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CR-11204 targets ENO1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENO1 fusion protein Ag1692
• Full Name: enolase 1, (alpha)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC015641
• Gene Symbol: ENO1
• Gene ID (NCBI): 2023
• Conjugate: Cardinal Red™ Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 592 nm / 611 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate.Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells .And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation.(19756395)