ENPP1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 87613-1-RR (same clone as 87613-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87613-1-RR (ENPP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87613-1-PBS in a different storage buffer formulation.

• 

  Affinity of 87613-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87657-1-RR against Human ENPP1 were performed. The affinity constant is 0.715 nM.

ENPP1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87613-1-PBS

Clone No.252965A5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Alkaline phosphodiesterase I, E NPP 1, Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, secreted form, E-NPP 1, M6S1

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Product Information

87613-1-PBS targets ENPP1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6489

  Product name: Recombinant human ENPP1 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 98-925 aa of NM_006208.2

  Sequence: KPSCAKEVKSCKGRCFERTFGNCRCDAACVELGNCCLDYQETCIEPEHIWTCNKFRCGEKRLTRSLCACSDDCKDKGDCCINYSSVCQGEKSWVEEPCESINEPQCPAGFETPPTLLFSLDGFRAEYLHTWGGLLPVISKLKKCGTYTKNMRPVYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPEWYKGEPIWVTAKYQGLKSGTFFWPGSDVEINGIFPDIYKMYNGSVPFEERILAVLQWLQLPKDERPHFYTLYLEEPDSSGHSYGPVSSEVIKALQRVDGMVGMLMDGLKELNLHRCLNLILISDHGMEQGSCKKYIYLNKYLGDVKNIKVIYGPAARLRPSDVPDKYYSFNYEGIARNLSCREPNQHFKPYLKHFLPKRLHFAKSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNVFSNMQALFVGYGPGFKHGIEADTFENIEVYNLMCDLLNLTPAPNNGTHGSLNHLLKNPVYTPKHPKEVHPLVQCPFTRNPRDNLGCSCNPSILPIEDFQTQFNLTVAEEKIIKHETLPYGRPRVLQKENTICLLSQHQFMSGYSQDILMPLWTSYTVDRNDSFSTEDFSNCLYQDFRIPLSPVHKCSFYKNNTKVSYGFLSPPQLNKNSSGIYSEALLTTNIVPMYQSFQVIWRYFHDTLLRKYAEERNGVNVVSGPVFDFDYDGRCDSLENLRQKRRVIRNQEILIPTHFFIVLTSCKDTSQTPLHCENLDTLAFILPHRTDNSESCVHGKHDSSWVEELLMLHRARITDVEHITGLSFYQQRKEPVSDILKLKTHLPTFSQED

• Full Name: ectonucleotide pyrophosphatase/phosphodiesterase 1
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 110-140 kD
• GenBank Accession Number: NM_006208.2
• Gene Symbol: ENPP1
• Gene ID (NCBI): 5167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P22413
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ENPP1, also known as PC1 or PC-1, is a type II transmembrane glycoprotein that serves as a critical enzymatic gatekeeper at the interface of cellular metabolism, mineral homeostasis, and immune signaling. It plays a crucial role in bone mineralization and soft tissue calcification, through hydrolyzing high-energy phosphate bonds to produce inorganic pyrophosphate (PPi), a potent inhibitor of ectopic mineral deposition (PMID: 37871131, PMID: 30799235, PMID: 40535334). ENPP1 inhibition protects cGAMP and ATP and reduces adenosine levels in the tumor microenvironment, activates antigen-presenting cells and increases T-cell infiltration promoting anticancer immunity (PMID: 40410143).