CD39/ENTPD1 Polyclonal antibody

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  WB analysis of mouse liver using 19229-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 19229-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19229-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19229-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19229-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19229-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19229-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19229-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19229-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 19229-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 19229-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19229-1-AP (CD39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 19229-1-AP

  IP result of anti-CD39/ENTPD1 (IP:19229-1-AP, 4ug; Detection:19229-1-AP 1:800) with mouse liver tissue lysate 4000ug.

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  IHC staining of human placenta using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19229-1-AP (CD39 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19229-1-AP (CD39 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 19229-1-AP (CD39 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human tonsillitis using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 19229-1-AP (CD39 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19229-1-AP (CD39 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19229-1-AP (CD39 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19229-1-AP (CD39 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 19229-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 19229-1-AP (CD39 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of human placenta using 19229-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD39/ENTPD1 antibody (19229-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human placenta using 19229-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD39/ENTPD1 antibody (19229-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CD39/ENTPD1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 19229-1-AP

Publications(10)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

ENTPD1, ATP diphosphohydrolase, ATPDase, ATP-DPH, CD39

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Tested Applications

• Positive WB detected in: mouse liver tissue, HL-60 cells, mouse brain tissue, human liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human placenta tissue, human liver tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

19229-1-AP targets CD39/ENTPD1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6165

  Product name: Recombinant human ENTPD1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-340 aa of BC047664

  Sequence: MESEELADRVLDVVERSLSNYPFDFQGARIITGQEEGAYGWITINYLLGKFSQKTRWFSIVPYETNNQETFGALDLGGASTQVTFVPQNQTIESPDNALQFRLYGKDYNVYTHSFLCYGKDQALWQKLAKDIQVASNEILRDPCFHPGYKKVVNVSDLYKTPCTKRFEMTLPFQQFEIQGIGNYQQCHQSILELFNTSYCPYSQCAFNGIFLPPLQGDFGAFSAFYFVMKFLNLTSEKVSQEKVTEMMKKFCAQPWEEIKTSYAGVKEKYLSEYCFSGTYILSLLLQGYHFTADSWEHIHFIGKIQGSDAGWTLGYMLNLTNMIPAEQPLSTPLSHSTYV

• Full Name: ectonucleoside triphosphate diphosphohydrolase 1
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 75-95 kDa
• GenBank Accession Number: BC047664
• Gene Symbol: CD39
• Gene ID (NCBI): 953
• RRID: AB_10859825
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49961
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). It has 3 isoforms produced by alternative splicing. CD39 preferentially occurred as a dimer that could be dissociated into monomeric forms. (PMID: 12694193). The soluble isoform of CD39(38-478 amino acid) is located in the extracellular part and 19229-1-AP recognizes both the 3 isoforms and the soluble form of CD39 (38-478aa) molecule.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Bone Res; Alpl prevents bone ageing sensitivity by specifically regulating senescence and differentiation in mesenchymal stem cells.; Authors - Wenjia Liu
human	WB	Biochim Biophys Acta Mol Basis Dis; Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages; Authors - Ling Zhou
human	IF	J Cell Mol Med; CD39 Contributes to the Ability of Cell Invasion in Heterogeneity of Colorectal Cancer; Authors - Xiaosong Li
human		J Chem Inf Model; Identification of Novel CD39 Inhibitors Based on Virtual Screening and Enzymatic Assays.; Authors - Yunshuo Zhao
rat	WB	Biochem J; Characterization of soluble CD39 (SolCD39/NTPDase1) from PiggyBac nonviral system as a tool to control the nucleotides level.; Authors - Liziane Raquel Beckenkamp
mouse	IHC	JCI Insight; Epithelial microRNA-206 targets CD39/extracellular ATP to upregulate airway IL-25 and TSLP in type 2-high asthma.; Authors - Kan Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Isabelle (Verified Customer) (10-08-2020)

good antibody for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: human liver, hepG2 cell line, renal tubular HK2 cell line