ENTPD5 Polyclonal antibody

ENTPD5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26746-1-AP

Synonyms

CD39 antigen like 4, CD39L4, ENTPD5, ER UDPase, NTPDase 5, Nucleoside diphosphatase, PCPH

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Validation Data Gallery

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  WB analysis of mouse liver using 26746-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26746-1-AP (ENTPD5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 26746-1-AP

  IP result of anti-ENTPD5 (IP:26746-1-AP, 4ug; Detection:26746-1-AP 1:300) with mouse liver tissue lysate 6000ug.

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  IHC staining of human liver using 26746-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26746-1-AP (ENTPD5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 26746-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26746-1-AP (ENTPD5 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26746-1-AP is ENTPD5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENTPD5 fusion protein Ag25022
• Full Name: ectonucleoside triphosphate diphosphohydrolase 5
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC130485
• Gene Symbol: ENTPD5
• Gene ID (NCBI): 957
• RRID: AB_2880619
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.