ENY2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ENY2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15778-1-AP

Synonyms

DC6, e(y)2, ENY2

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Validation Data Gallery

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  WB analysis of PC-3 using 15778-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15778-1-AP (ENY2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 15778-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15778-1-AP (ENY2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15778-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15778-1-AP (ENY2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Product Information

15778-1-AP targets ENY2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENY2 fusion protein Ag8462
• Full Name: enhancer of yellow 2 homolog (Drosophila)
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC007870
• Gene Symbol: ENY2
• Gene ID (NCBI): 56943
• RRID: AB_2878184
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENY2, also named as DC6, is involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. It is required for nuclear receptor-mediated transactivation. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). The MW of ENY2 is about 10 kDa.

Publications

Species	Application	Title
human	WB, IP	J Virol; The cellular protein complex associated with a transforming region of E1A contains c-MYC.; Authors - S Vijayalingam ; KD Validated