|Positive WB detected in||HeLa cells, HepG2 cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
66731-1-Ig targets EPAS1 in WB, IHC,ELISA applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / IgG2a|
|Immunogen||EPAS1 fusion protein Ag24886|
|Full Name||endothelial PAS domain protein 1|
|Calculated molecular weight||96 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC051338|
|Gene ID (NCBI)||2034|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Endothelial PAS domain-containing protein 1 (EPAS1), also known as Hypoxia-inducible factor 2-alpha (HIF2-alpha,HIF2A), is a transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. EPAS1 is expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
lUNFENG (Verified Customer) (01-27-2020)
IT IS NOT VERY GOOD.
Jie (Verified Customer) (01-27-2020)
Positive signal in heart samples, however, does not appear to be decreased in our KO mice model.