EPHA1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of HepG2 using 87362-1-RR (same clone as 87362-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 87362-1-RR (EPHA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87362-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 87362-1-RR (same clone as 87362-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 87362-1-RR (EPHA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87362-1-PBS in a different storage buffer formulation.

• 

  Affinity of 87362-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87362-1-RR against Human EPHA1 were performed. The affinity constant is 1.22 nM.

EPHA1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87362-1-PBS

Clone No.252393D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

EPHA1-special, EC:2.7.10.1, EPH, EPH receptor A1, EPH tyrosine kinase

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Product Information

87362-1-PBS targets EPHA1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5474

  Product name: Recombinant human EPHA1 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 26-547 aa of NM_005232.4

  Sequence: KEVTLMDTSKAQGELGWLLDPPKDGWSEQQQILNGTPLYMYQDCPMQGRRDTDHWLRSNWIYRGEEASRVHVELQFTVRDCKSFPGGAGPLGCKETFNLLYMESDQDVGIQLRRPLFQKVTTVAADQSFTIRDLVSGSVKLNVERCSLGRLTRRGLYLAFHNPGACVALVSVRVFYQRCPETLNGLAQFPDTLPGPAGLVEVAGTCLPHARASPRPSGAPRMHCSPDGEWLVPVGRCHCEPGYEEGGSGEACVACPSGSYRMDMDTPHCLTCPQQSTAESEGATICTCESGHYRAPGEGPQVACTGPPSAPRNLSFSASGTQLSLRWEPPADTGGRQDVRYSVRCSQCQGTAQDGGPCQPCGVGVHFSPGARGLTTPAVHVNGLEPYANYTFNVEAQNGVSGLGSSGHASTSVSISMGHAESLSGLSLRLVKKEPRQLELTWAGSRPRSPGANLTYELHVLNQDEERYQMVLEPRVLLTELQPDTTYIVRVRMLTPLGPGPFSPDHEFRTSPPVSRGLTGGE

• Full Name: EPH receptor A1
• Calculated Molecular Weight: 108 kDa
• Observed Molecular Weight: 120-125 kDa
• GenBank Accession Number: NM_005232.4
• Gene Symbol: EPHA1
• Gene ID (NCBI): 2041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21709-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EPHA1, also named as EPH, EPHT and EPHT1, belongs to ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositol linkages, whereas ephrin-B subclass consists of ligands with transmembrane domains. The general role of the Eph family is in mediating repulsive cell-cell interaction.