ESAM Recombinant monoclonal antibody

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  WB analysis using 87684-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87684-1-RR (ESAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ESAM Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87684-1-RR

Clone No.253062C8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Endothelial cell-selective adhesion molecule, Esam1

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Tested Applications

• Positive WB detected in: bEnd.3 cells, mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87684-1-RR targets ESAM in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6352

  Product name: Recombinant mouse ESAM protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 30-251 aa of NM_027102.3

  Sequence: QMELHVPPGLNKLEAVEGEEVVLPAWYTMAREESWSHPREVPILIWFLEQEGKEPNQVLSYINGVMTNKPGTALVHSISSRNVSLRLGALQEGDSGTYRCSVNVQNDEGKSIGHSIKSIELKVLVPPAPPSCSLQGVPYVGTNVTLNCKSPRSKPTAQYQWERLAPSSQVFFGPALDAVRGSLKLTNLSIAMSGVYVCKAQNRVGFAKCNVTLDVMTGSKAA

• Full Name: endothelial cell-specific adhesion molecule
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42-60 kDa
• GenBank Accession Number: NM_027102.3
• Gene Symbol: Esam
• Gene ID (NCBI): 69524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q925F2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ESAM, a 55 kDa type I transmembrane glycoprotein, possesses two extracellular Ig domains and a long cytoplasmic tail in which a PDZ domain is located (PMID: 22130718). The ~60 kDa band is glycosylated ESAM, and the lower band is deglycosylated ESAM.