ESS2/DGCR14 Polyclonal antibody

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  WB analysis using 33574-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33574-1-AP ( DGCR14 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

ESS2/DGCR14 Polyclonal Antibody for WB, ELISA

Cat No. 33574-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DGCR14, ESS2, DGCR13, DGSH, DGS-H

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Tested Applications

• Positive WB detected in: DU 145 cells, HEK-293 cells, LNCaP cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33574-1-AP targets ESS2/DGCR14 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag37915

  Product name: Recombinant human ESS2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-250 aa of NM_022719

  Sequence: METPGASASSLLLPAASRPPRKREAGEAGAATSKQRVLDEEEYIEGLQTVIQRDFFPDVEKLQAQKEYLEAEENGDLERMRQIAIKFGSALGKMSREPPPPYVTPATFETPEVHAGTGVVGNKPRPRGRGLEDGEAGEEEEKEPLPSLDVFLSRYTSEDNASFQEIMEVAKERSRARHAWLYQAEEEFEKRQKDNLELPSAEHQAIESSQASVETWKYKAKNSLMYYPEGVPDEEQLFKKPRQVVHKNTR

• Full Name: DiGeorge syndrome critical region gene 14
• Calculated Molecular Weight: 476aa, 53 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: NM_022719
• Gene Symbol: DGCR14
• Gene ID (NCBI): 8220
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q96DF8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ESS2 splicing factor homolog (ESS2), also named DiGeorge-syndrome critical region 14 (DGCR14), acts as a transcriptional co-activator for retinoic acid receptor-related orphan nuclear receptor gamma/gamma-t (Rorγ/γt). Ess2 is a nuclear protein with a coiled-coil domain [6] that is conserved from yeasts to humans. ESS2 is a member of the deletion gene cluster in the 22q11.2 deletion syndrome (22q11.2DS, also known as DiGeorge syndrome or CATCH 22 syndrome). The syndrome is characterized by varying defects of the heart, thymus and parathyroid glands.