Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, human heart tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach cancer tissue, rat heart tissue, rat brain tissue, mouse heart tissue, human colon cancer tissue, human placenta tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17925-1-AP targets ETFB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12344 Product name: Recombinant human ETFB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-255 aa of BC093961 Sequence: MAELRVLVAVKRVIDYAVKIRVKPDRTGVVTDGVKHSMNPFCEIAVEEAVRLKEKKLVKEVIAVSCGPAQCQETIRTALAMGADRGIHVEVPPAEAERLGPLQVARVLAKLAEKEKVDLVLLGKQAIDDDCNQTGQMTAGFLDWPQGTFASQVTLEGDKLKVEREIDGGLETLRLKLPAVVTADLRLNEPRYATLPNIMKAKKKKIEVIKPGDLGVDLTSKLSVISVEDPPQRTAGVKVETTEDLVAKLKEIGRI Predict reactive species |
| Full Name | electron-transfer-flavoprotein, beta polypeptide |
| Calculated Molecular Weight | 255 aa, 28 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC093961 |
| Gene Symbol | ETFB |
| Gene ID (NCBI) | 2109 |
| RRID | AB_2100560 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P38117 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Electron transfer flavoprotein (ETF) exists in the mitochondrial matrix as a heterodimer of 30 kDa alpha subunits (ETFA)and 28 kDa beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer.It serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase.ETFB has 2 isoforms produced by alternative splicing. Defects in ETFB are the cause of glutaric aciduria type 2B (GA2B).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ETFB antibody 17925-1-AP | Download protocol |
| IHC protocol for ETFB antibody 17925-1-AP | Download protocol |
| IP protocol for ETFB antibody 17925-1-AP | Download protocol |
| WB protocol for ETFB antibody 17925-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-ETFB polyclonal antibody (Cat# 17925-1-AP) has 11 citations. These appear in journals including Molecular Cell, Developmental Cell, Journal of Cellular and Molecular Medicine, iScience, Insect Biochemistry and Molecular Biology, Journal of Biological Chemistry, Journal of Cell Science, FEBS Letters, Molecular and Cellular Biology, and Journal of International Medical Research. Research fields span mitochondrial biology, electron transfer flavoprotein function, protein methylation, oxidative stress, apoptosis, ferroptosis, senescence, and mitochondrial dysfunction in cardiomyocytes. Applications cited include WB and IF across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. | ||
J Cell Mol Med New protein-protein interactions of mitochondrial connexin 43 in mouse heart. | ||
iScience Mitochondrial hyper-acetylation induced by an engineered acetyltransferase promotes cellular senescence | ||
Insect Biochem Mol Biol Energy production is suppressed by vcircRNA_000048- bom-miR-2753-METTL20 regulatory axis in the BmCPV-infected silkworm, Bombyx mori. |
Reviews
What customers say
The single review reports a 5-star rating for this antibody used in Western Blot on Brown Adipocytes. The user found it works well at a 1:2000 dilution, indicating reliable performance for their application. Overall, the feedback is positive, with the reviewer confirming effective results in their specific experimental setup.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Valentin (Verified Customer) (04-14-2026) | Works well for western blot in 1:2000 dilution
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