ETFB Polyclonal antibody

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  WB analysis of mouse skeletal muscle using 17925-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 17925-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 17925-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 17925-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17925-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 17925-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17925-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17925-1-AP (ETFB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17925-1-AP

  IP result of anti-ETFB (IP:17925-1-AP, 3ug; Detection:17925-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human stomach cancer using 17925-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17925-1-AP (ETFB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17925-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ETFB antibody (17925-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

ETFB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17925-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Beta-ETF, FP585, MADD

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human heart tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 2 publications below

Product Information

17925-1-AP targets ETFB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12344

  Product name: Recombinant human ETFB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-255 aa of BC093961

  Sequence: MAELRVLVAVKRVIDYAVKIRVKPDRTGVVTDGVKHSMNPFCEIAVEEAVRLKEKKLVKEVIAVSCGPAQCQETIRTALAMGADRGIHVEVPPAEAERLGPLQVARVLAKLAEKEKVDLVLLGKQAIDDDCNQTGQMTAGFLDWPQGTFASQVTLEGDKLKVEREIDGGLETLRLKLPAVVTADLRLNEPRYATLPNIMKAKKKKIEVIKPGDLGVDLTSKLSVISVEDPPQRTAGVKVETTEDLVAKLKEIGRI

• Full Name: electron-transfer-flavoprotein, beta polypeptide
• Calculated Molecular Weight: 255 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC093961
• Gene Symbol: ETFB
• Gene ID (NCBI): 2109
• RRID: AB_2100560
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P38117
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Electron transfer flavoprotein (ETF) exists in the mitochondrial matrix as a heterodimer of 30 kDa alpha subunits (ETFA)and 28 kDa beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer.It serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase.ETFB has 2 isoforms produced by alternative splicing. Defects in ETFB are the cause of glutaric aciduria type 2B (GA2B).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Mol Cell; PrimPol, an archaic primase/polymerase operating in human cells.; Authors - Sara García-Gómez
human	WB	Dev Cell; Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation; Authors - Wanxin Guo
human	WB	Mol Cell Biol; A mutation in the FAD-dependent oxidoreductase FOXRED1 results in cell-type specific assembly defects in oxidative phosphorylation complexes I and II.; Authors - Olga Zurita Rendón
human	WB	J Biol Chem; Human METTL20 Methylates Lysine Residues Adjacent to the Recognition Loop of the Electron Transfer Flavoprotein in Mitochondria.; Authors - Virginie F Rhein
human	WB	J Cell Sci; Mitochondrial maintenance under oxidative stress depends on mitochondrial but not nuclear α isoform of OGG1.; Authors - Debora Lia