ETFDH Polyclonal antibody

• Featured Product
• KD/KO Validated

ETFDH Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 11109-1-AP

Synonyms

ETF dehydrogenase, ETF QO, ETF ubiquinone oxidoreductase, ETFDH, ETFQO, MADD

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Validation Data Gallery

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  WB analysis using 11109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11109-1-AP

  WB result of ETFDH antibody (11109-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ETFDH transfected HEK-293 cells.

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  WB analysis of HEK-293 using 11109-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11109-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11109-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11109-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11109-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11109-1-AP (ETFDH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11109-1-AP

  IP result of anti-ETFDH (IP:11109-1-AP, 3ug; Detection:11109-1-AP 1:500) with mouse brain tissue lysate 8000ug.

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  IHC staining of human liver cancer using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11109-1-AP (ETFDH antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11109-1-AP (ETFDH antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11109-1-AP (ETFDH antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11109-1-AP (ETFDH antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11109-1-AP (ETFDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells, rat brain tissue, mouse kidney tissue, mouse skeletal muscle tissue, rat kidney tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human liver cancer tissue, human liver tissue, human kidney tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

11109-1-AP targets ETFDH in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ETFDH fusion protein Ag1568
• Full Name: electron-transferring-flavoprotein dehydrogenase
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC011890
• Gene Symbol: ETFDH
• Gene ID (NCBI): 2110
• RRID: AB_2231382
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ETFDH(Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial) is also named as ETF-QO and belongs to the ETF-QO/fixC family.It is a 64-kDa monomer integrated in the inner mitochondrial membrane, con- tains one molecule of FAD and a 4Fe4S cluster(PMID:12815589).Defects in ETFDH are the cause of glutaric aciduria type 2C (GA2C).This antibody is speicific to ETFDH.

Publications

Species	Application	Title
human,mouse	WB	Ann Neurol; Both ETFDH mutations and FAD homeostasis disturbance are essential for developing riboflavin-responsive multiple Acyl-CoA dehydrogenation deficiency.; Authors - Jingwen Xu
mouse	WB	Cell Mol Life Sci; Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.; Authors - Min-Zhi Peng
human	WB	Hum Mol Genet; Clinical, pathological and genetic features and follow-up of 110 patients with late-onset MADD: A single-center retrospective study.; Authors - Bing Wen
human	WB	J Neurol Neurosurg Psychiatry; Riboflavin-responsive lipid-storage myopathy caused by ETFDH gene mutations.; Authors - Wen Bing B
human	WB,IF	Front Oncol; Propofol Inhibits Proliferation and Augments the Anti-Tumor Effect of Doxorubicin and Paclitaxel Partly Through Promoting Ferroptosis in Triple-Negative Breast Cancer Cells.; Authors - Chen Sun
human	WB	J Mol Med (Berl); Molecular analysis of 51 unrelated pedigrees with late-onset multiple acyl-CoA dehydrogenation deficiency (MADD) in southern China confirmed the most common ETFDH mutation and high carrier frequency of c.250G>A.; Authors - Wang Zhi-Qiang ZQ