EWS Polyclonal antibody, PBS Only

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  WB analysis using 55191-1-AP (same clone as 55191-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 55191-1-AP (same clone as 55191-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 55191-1-AP (same clone as 55191-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 55191-1-AP (same clone as 55191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 55191-1-AP (same clone as 55191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 55191-1-AP (same clone as 55191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 55191-1-AP (same clone as 55191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

EWS Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 55191-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

EWSR1, EWS oncogene, RNA binding protein EWS, RNA-binding protein EWS

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Product Information

55191-1-PBS targets EWS in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Ewing sarcoma breakpoint region 1
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: NM_005243
• Gene Symbol: EWS
• Gene ID (NCBI): 2130
• RRID: AB_10860907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q01844
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EWS, also named as WSR1, belongs to the RRM TET family. It might normally function as a repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. This antibody is specific to EWS. And a 90 kDa band also could be detected (PMID:23552423).