Validation Data Gallery
|Positive WB detected in
|K-562 cells, mouse testis tissue, Jurkat cells
|Positive IHC detected in
|human colon cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:1000-1:6000
|IHC : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
55191-1-AP targets EWS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|Ewing sarcoma breakpoint region 1
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
EWS, also named as WSR1, belongs to the RRM TET family. It might normally function as a repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. This antibody is specific to EWS. And a 90 kDa band also could be detected (PMID:23552423).
Nat Struct Mol Biol
TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.
Nucleic Acids Res
The RNA binding protein EWS is broadly involved in the regulation of pri-miRNA processing in mammalian cells.
LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation
Mol Ther Nucleic Acids
Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Manohar (Verified Customer) (12-18-2021)
Overnight primary antibody using milk to block the blot.