Validation Data Gallery
|Positive WB detected in||K-562 cells, mouse testis tissue, Jurkat cells, human testis tissue|
|Positive IHC detected in||human colon cancer tissue, human small intestine tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
55191-1-AP targets EWS in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||Ewing sarcoma breakpoint region 1|
|Calculated molecular weight||68 kDa|
|Observed molecular weight||90 kDa|
|GenBank accession number||NM_005243|
|Gene ID (NCBI)||2130|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
EWS, also named as WSR1, belongs to the RRM TET family. It might normally function as a repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. This antibody is specific to EWS.And a 90 kDa band also could be detected (PMID:23552423).
Nucleic Acids Res
The RNA binding protein EWS is broadly involved in the regulation of pri-miRNA processing in mammalian cells.
Mol Ther Nucleic Acids
Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.
Nat Struct Mol Biol
TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Manohar (Verified Customer) (12-18-2021)
Overnight primary antibody using milk to block the blot.