EWS Polyclonal antibody

EWS Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55191-1-AP

Synonyms

EWS, EWS oncogene, EWSR1, RNA binding protein EWS



Tested Applications

Positive WB detected inK-562 cells, mouse testis tissue, Jurkat cells
Positive IHC detected inhuman colon cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

55191-1-AP targets EWS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Ewing sarcoma breakpoint region 1
Calculated molecular weight 68 kDa
Observed molecular weight68-70 kDa
GenBank accession numberNM_005243
Gene symbol EWSR1
Gene ID (NCBI) 2130
RRIDAB_10860907
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EWS, also named as WSR1, belongs to the RRM TET family. It might normally function as a repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. This antibody is specific to EWS. And a 90 kDa band also could be detected (PMID:23552423).

Protocols

Product Specific Protocols
WB protocol for EWS antibody 55191-1-APDownload protocol
IHC protocol for EWS antibody 55191-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
humanWB

Nucleic Acids Res

The RNA binding protein EWS is broadly involved in the regulation of pri-miRNA processing in mammalian cells.

Authors - Huiwu Ouyang
humanWB

Cell Rep

LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation

Authors - Nasha Zhang
ratWB

Mol Ther Nucleic Acids

Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.

Authors - Zhijie Shen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Manohar (Verified Customer) (12-18-2021)

Overnight primary antibody using milk to block the blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293