EXOSC9 Monoclonal antibody

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  WB analysis using 67636-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67636-1-Ig (EXOSC9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HeLa using 67636-1-Ig

  WB result of EXOSC9 antibody (67636-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC9 transfected HeLa cells.

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  IHC staining of human breast cancer using 67636-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 67636-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 67636-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67636-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67636-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC9 antibody (67636-1-Ig, Clone: 3F4G1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

EXOSC9 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67636-1-Ig

Clone No.3F4G1

Publications(1)

Reviews (1)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Autoantigen PM/Scl 1, EXOSC9, exosome component 9, p5, p6, PM/Scl 75, PMSCL1, RRP45, Rrp45p

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Tested Applications

• Positive WB detected in: U2OS cells, HeLa cells, A549 cells, MCF-7 cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue, mouse kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:750-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67636-1-Ig targets EXOSC9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19783

  Product name: Recombinant human EXOSC9 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-351 aa of BC142978

  Sequence: MKETPLSNCERRFLLRAIEEKKRLDGRQTYDYRNIRISFGTDYGCCIVELGKTRVLGQVSCELVSPKLNRATEGILFFNLELSQMAAPAFEPGRQSDLLVKLNRLMERCLRNSKCIDTESLCVVAGEKVWQIRVDLHLLNHDGNIIDAASIAAIVALCHFRRPDVSVQGDEVTLYTPEERDPVPLSIHHMPICVSFAFFQQGAYLLVDPNEREERVMDGLLVIAMNKHREICTIQSSGGIMLLKDQVLRCSKIAGVKVAEITELILKALENDQKVRKEGGKFGFAESIANQRITAFKMEKAPIDTSDVEEKAEEIIAEAEPPSEVVSTPVLWTPGTAQIGEGVENSWGDLE

• Full Name: exosome component 9
• Calculated Molecular Weight: 456 aa, 51 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC142978
• Gene Symbol: EXOSC9
• Gene ID (NCBI): 5393
• RRID: AB_2882837
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q06265
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Rep; Cytoplasmic DIS3 is an exosome-independent endoribonuclease with catalytic activity toward circular RNAs; Authors - Claudia Latini

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nikolaus (Verified Customer) (07-14-2022)

Antibodies were incubated ON at 4C. Exosc9 depletion was used as a control to check the specificity of the band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC9 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:67636-1-Ig)