EXOSC9 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 67636-1-Ig (same clone as 67636-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67636-1-Ig (EXOSC9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67636-1-Ig (same clone as 67636-1-PBS)

  WB result of EXOSC9 antibody (67636-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC9 transfected HeLa cells. This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67636-1-Ig (same clone as 67636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67636-1-Ig (same clone as 67636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 67636-1-Ig (same clone as 67636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67636-1-Ig (same clone as 67636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67636-1-Ig (same clone as 67636-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC9 antibody (67636-1-Ig, Clone: 3F4G1 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51449-1

  Cytometric bead array sample test of MP51449-1, EXOSC9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67636-1-PBS. Detection antibody: 67636-2-PBS.

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  Cytometric bead array standard curve of MP51449-1

  Cytometric bead array standard curve of MP51449-1, EXOSC9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67636-1-PBS. Detection antibody: 67636-2-PBS. Standard:Ag19783. Range: 3.125-400 ng/mL.

• Featured Product
• KD/KO Validated

EXOSC9 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 67636-1-PBS

Clone No.3F4G1

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

3F4G1, Exosome complex component RRP45, P75 polymyositis-scleroderma overlap syndrome-associated autoantigen, PM/Scl-75, PMSCL1

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Product Information

67636-1-PBS targets EXOSC9 as part of a matched antibody pair:

MP51449-1: 67636-1-PBS capture and 67636-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19783

  Product name: Recombinant human EXOSC9 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-351 aa of BC142978

  Sequence: MKETPLSNCERRFLLRAIEEKKRLDGRQTYDYRNIRISFGTDYGCCIVELGKTRVLGQVSCELVSPKLNRATEGILFFNLELSQMAAPAFEPGRQSDLLVKLNRLMERCLRNSKCIDTESLCVVAGEKVWQIRVDLHLLNHDGNIIDAASIAAIVALCHFRRPDVSVQGDEVTLYTPEERDPVPLSIHHMPICVSFAFFQQGAYLLVDPNEREERVMDGLLVIAMNKHREICTIQSSGGIMLLKDQVLRCSKIAGVKVAEITELILKALENDQKVRKEGGKFGFAESIANQRITAFKMEKAPIDTSDVEEKAEEIIAEAEPPSEVVSTPVLWTPGTAQIGEGVENSWGDLE

• Full Name: exosome component 9
• Calculated Molecular Weight: 456 aa, 51 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC142978
• Gene Symbol: EXOSC9
• Gene ID (NCBI): 5393
• RRID: AB_2882837
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q06265
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.