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EZH2 Polyclonal antibody
EZH2 Polyclonal Antibody for FC, IF, IP, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (1)
WB, RIP, IP, IF, FC, CoIP, ChIP, ELISA
Cat no : 21800-1-AP
Validation Data Gallery
|Positive IP detected in||HepG2 cells|
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21800-1-AP is EZH2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EZH2 fusion protein Ag16789|
|Full Name||enhancer of zeste homolog 2 (Drosophila)|
|Calculated molecular weight||751 aa, 86 kDa|
|Observed molecular weight||90-102 kDa|
|GenBank accession number||BC010858|
|Gene ID (NCBI)||2146|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)
|Product Specific Protocols|
|WB protocol for EZH2 antibody 21800-1-AP||Download protocol|
|IF protocol for EZH2 antibody 21800-1-AP||Download protocol|
|IP protocol for EZH2 antibody 21800-1-AP||Download protocol|
|Click here to view our Standard Protocols|
VE-Cadherin-Mediated Epigenetic Regulation of Endothelial Gene Expression.
Nat Struct Mol Biol
lnc-β-Catm elicits EZH2-dependent β-catenin stabilization and sustains liver CSC self-renewal.
CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression.
Clin Cancer Res
Single-Cell Analyses Reveal Mechanisms of Cancer Stem Cell Maintenance and Epithelial-Mesenchymal Transition in Recurrent Bladder Cancer.
PRMT5 functionally associates with EZH2 to promote colorectal cancer progression through epigenetically repressing CDKN2B expression.
1,25-Dihydroxyvitamin D protects against age-related osteoporosis by a novel VDR-Ezh2-p16 signal axis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Nikhil (Verified Customer) (10-16-2019)