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EZH2 Polyclonal antibody
EZH2 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IF, chIP, ELISA
Conjugate
Unconjugated
Cat no : 21800-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | DU 145 cells, HEK-293 cells, mouse kidney tissue, rat kidney tissue, Jurkat cells, BxPC-3 cells, Raji cells, NIH/3T3 cells, A431 cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 33 publications below |
| IHC | See 5 publications below |
| IF | See 4 publications below |
| ChIP | See 2 publications below |
Product Information
The immunogen of 21800-1-AP is EZH2 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EZH2 fusion protein Ag16789 |
| Full Name | enhancer of zeste homolog 2 (Drosophila) |
| Calculated molecular weight | 751 aa, 86 kDa |
| Observed molecular weight | 90-102 kDa |
| GenBank accession number | BC010858 |
| Gene symbol | EZH2 |
| Gene ID (NCBI) | 2146 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EZH2 antibody 21800-1-AP | Download protocol |
| IF protocol for EZH2 antibody 21800-1-AP | Download protocol |
| IP protocol for EZH2 antibody 21800-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis CUL1 promotes breast cancer metastasis through regulating EZH2-induced the autocrine expression of the cytokines CXCL8 and IL11. | ||
Int J Biol Sci Identification and Functional Characterization of a New Splicing Variant of EZH2 in the Central Nervous System. | ||
Biochim Biophys Acta Gene Regul Mech Epigenetic silencing of the synthesis of immunosuppressive Siglec ligand glycans by NF-κB/EZH2/YY1 axis in early-stage colon cancers. | ||
Oncogene MDM2 promotes genome instability by ubiquitinating the transcription factor HBP1. | ||
Theriogenology Effect of EZH2 knockdown on preimplantation development of porcine parthenogenetic embryos. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Nikhil (Verified Customer) (10-16-2019) | very nice
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