EZH2 Polyclonal antibody

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  WB analysis using 21800-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 21800-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 21800-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 21800-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 21800-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21800-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21800-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21800-1-AP

  Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 21800-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21800-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 21800-1-AP

  IP result of anti-EZH2 (IP:21800-1-AP, 4ug; Detection:21800-1-AP 1:1000) with HepG2 cells lysate 3400ug.

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  IHC staining of human lung squamous cell carcinoma using 21800-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 21800-1-AP (EZH2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21800-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EZH2 antibody (21800-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 21800-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human EZH2 (21800-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

EZH2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 21800-1-AP

Publications(104)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Synonyms

KMT6, EZH1, ENX-1, ENX 1, Enhancer of zeste homolog 2

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Tested Applications

• Positive WB detected in: DU 145 cells, HEK-293 cells, mouse kidney tissue, NIH/3T3 cells, Raji cells, Jurkat cells, A431 cells, HepG2 cells, rat kidney tissue, C6 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human lung squamous cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

This antibody is not recommended for use in IHC.

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 21 publications below
• WB: See 90 publications below
• IF: See 11 publications below
• IP: See 4 publications below
• CoIP: See 4 publications below
• ChIP: See 11 publications below
• RIP: See 1 publications below

Product Information

21800-1-AP targets EZH2 in WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EZH2 fusion protein Ag16789
• Full Name: enhancer of zeste homolog 2 (Drosophila)
• Calculated Molecular Weight: 751 aa, 86 kDa
• Observed Molecular Weight: 90-102 kDa
• GenBank Accession Number: BC010858
• Gene Symbol: EZH2
• Gene ID (NCBI): 2146
• RRID: AB_10858790
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15910
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)

Publications

Species	Application	Title
human	WB	Mol Cancer; Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer; Authors - Rashmi Bharti
human	WB	Circ Res; VE-Cadherin-Mediated Epigenetic Regulation of Endothelial Gene Expression.; Authors - Marco F Morini
human	WB,IF	Nat Struct Mol Biol; lnc-β-Catm elicits EZH2-dependent β-catenin stabilization and sustains liver CSC self-renewal.; Authors - Pingping Zhu
		Cancer Res; HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc.; Authors - Yinghui Li
human,mouse	WB	Theranostics; Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI; Authors - Bojun Li ; KD Validated
human	WB	Nat Commun; CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression.; Authors - Yongqing Liu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nikhil (Verified Customer) (10-16-2019)

very nice

• Applications: Western Blot,
• Cell Tissue Type: Colon cancer cells

EZH2 Antibody Western Blot, validation ( dilution) in Colon cancer cells (Cat no:21800-1-AP)