- Featured Product
- KD/KO Validated
EZH2 Polyclonal antibody
EZH2 Polyclonal Antibody for FC, IF, IP, WB, ELISA
Cat no : 21800-1-AP
Validation Data Gallery
|Positive WB detected in||DU 145 cells, HEK-293 cells, mouse kidney tissue, NIH/3T3 cells, Raji cells, Jurkat cells, A431 cells, HepG2 cells, rat kidney tissue, C6 cells|
|Positive IP detected in||HepG2 cells|
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
21800-1-AP targets EZH2 in WB, RIP, IP, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, sheep, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EZH2 fusion protein Ag16789|
|Full Name||enhancer of zeste homolog 2 (Drosophila)|
|Calculated molecular weight||751 aa, 86 kDa|
|Observed molecular weight||90-102 kDa|
|GenBank accession number||BC010858|
|Gene ID (NCBI)||2146|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)
VE-Cadherin-Mediated Epigenetic Regulation of Endothelial Gene Expression.
Nat Struct Mol Biol
lnc-β-Catm elicits EZH2-dependent β-catenin stabilization and sustains liver CSC self-renewal.
HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc.
Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI
CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression.
Clin Cancer Res
Single-Cell Analyses Reveal Mechanisms of Cancer Stem Cell Maintenance and Epithelial-Mesenchymal Transition in Recurrent Bladder Cancer.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Nikhil (Verified Customer) (10-16-2019)