EZH2 Polyclonal antibody

• Featured Product
• KD/KO Validated

EZH2 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, FC, CHIP, ELISA

Conjugate

Unconjugated

Publications

57

Reviews (1)

Cat no : 21800-1-AP

Synonyms

Enhancer of zeste homolog 2, ENX 1, ENX-1, EZH1, EZH2, KMT6, Lysine N methyltransferase 6

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Validation Data Gallery

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  WB analysis of DU 145 using 21800-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21800-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21800-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 21800-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 21800-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21800-1-AP

  Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 21800-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21800-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21800-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21800-1-AP (EZH2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 21800-1-AP

  IP Result of anti-EZH2 (IP:21800-1-AP, 4ug; Detection:21800-1-AP 1:1000) with HepG2 cells lysate 3400ug.

• 

  IF Staining of HepG2 using 21800-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 21800-1-AP (EZH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 21800-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human EZH2 (21800-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Tested Applications

• Positive WB detected in: DU 145 cells, A431 cells, HEK-293 cells, HepG2 cells, Jurkat cells, mouse kidney tissue, NIH/3T3 cells, Raji cells, rat kidney tissue
• Positive IP detected in: HepG2 cells
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Planning an IF experiment? We recommend our CoraLite®488, CoraLite®555 and CoraLite®650 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 15 publications below
• WB: See 51 publications below
• IHC: See 9 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• ChIP: See 3 publications below

Product Information

The immunogen of 21800-1-AP is EZH2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EZH2 fusion protein Ag16789
• Full Name: enhancer of zeste homolog 2 (Drosophila)
• Calculated molecular weight: 751 aa, 86 kDa
• Observed molecular weight: 90-102 kDa
• GenBank accession number: BC010858
• Gene symbol: EZH2
• Gene ID (NCBI): 2146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)

Publications

Species	Application	Title
human	WB,IHC	Cell Death Dis; CUL1 promotes breast cancer metastasis through regulating EZH2-induced the autocrine expression of the cytokines CXCL8 and IL11.; Authors - Ye-Fei Huang
rat	WB,IF	Int J Biol Sci; Identification and Functional Characterization of a New Splicing Variant of EZH2 in the Central Nervous System.; Authors - Danyang Li
		Biochim Biophys Acta Gene Regul Mech; Epigenetic silencing of the synthesis of immunosuppressive Siglec ligand glycans by NF-κB/EZH2/YY1 axis in early-stage colon cancers.; Authors - Hsiang-Chi Huang
human	WB	Oncogene; MDM2 promotes genome instability by ubiquitinating the transcription factor HBP1.; Authors - Zhengyi Cao
human	WB	Mol Ther Nucleic Acids; PMID: 30925453; Authors - Gan Yu ; KD Validated
pig	WB	Theriogenology; Effect of EZH2 knockdown on preimplantation development of porcine parthenogenetic embryos.; Authors - Qingqing Cai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nikhil (Verified Customer) (10-16-2019)

very nice

• Applications: Western Blot,
• Cell Tissue Type: Colon cancer cells

EZH2 Antibody Western Blot, validation ( dilution) in Colon cancer cells (Cat no:21800-1-AP)

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