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  • KD/KO Validated

EZH2 Polyclonal antibody

EZH2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, chIP, ELISA

Conjugate

Unconjugated

Cat no : 21800-1-AP

Synonyms

Enhancer of zeste homolog 2, ENX 1, EZH1, EZH2, KMT6, Lysine N methyltransferase 6



Tested Applications

Positive WB detected inDU 145 cells, HEK-293 cells, mouse kidney tissue, rat kidney tissue, Jurkat cells, BxPC-3 cells, Raji cells, NIH/3T3 cells, A431 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21800-1-AP is EZH2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EZH2 fusion protein Ag16789
Full Name enhancer of zeste homolog 2 (Drosophila)
Calculated molecular weight 751 aa, 86 kDa
Observed molecular weight 90-102 kDa
GenBank accession numberBC010858
Gene symbol EZH2
Gene ID (NCBI) 2146
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)

Protocols

Product Specific Protocols
WB protocol for EZH2 antibody 21800-1-APDownload protocol
IF protocol for EZH2 antibody 21800-1-APDownload protocol
IP protocol for EZH2 antibody 21800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

CUL1 promotes breast cancer metastasis through regulating EZH2-induced the autocrine expression of the cytokines CXCL8 and IL11.

Authors - Ye-Fei Huang
ratWB,IF

Int J Biol Sci

Identification and Functional Characterization of a New Splicing Variant of EZH2 in the Central Nervous System.

Authors - Danyang Li

Biochim Biophys Acta Gene Regul Mech

Epigenetic silencing of the synthesis of immunosuppressive Siglec ligand glycans by NF-κB/EZH2/YY1 axis in early-stage colon cancers.

Authors - Hsiang-Chi Huang
humanWB

Oncogene

MDM2 promotes genome instability by ubiquitinating the transcription factor HBP1.

Authors - Zhengyi Cao
humanWB

Mol Ther Nucleic Acids

PMID: 30925453

Authors - Gan Yu
  • KD Validated
pigWB

Theriogenology

Effect of EZH2 knockdown on preimplantation development of porcine parthenogenetic embryos.

Authors - Qingqing Cai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Nikhil (Verified Customer) (10-16-2019)

very nice

  • Applications: Western Blot,
  • Cell Tissue Type: Colon cancer cells
EZH2 Antibody Western Blot, validation ( dilution) in Colon cancer cells (Cat no:21800-1-AP)