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CoraLite®488-conjugated EZH2 Polyclonal antibody

EZH2 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®488 Fluorescent Dye

Cat no : CL488-21800

Synonyms

Enhancer of zeste homolog 2, ENX 1, EZH1, EZH2, KMT6, Lysine N methyltransferase 6



Tested Applications

Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 21800-1-AP

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-21800 is EZH2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EZH2 fusion protein Ag16789
Full Name enhancer of zeste homolog 2 (Drosophila)
Calculated molecular weight 751 aa, 86 kDa
Observed molecular weight 90-102 kDa
GenBank accession numberBC010858
Gene symbol EZH2
Gene ID (NCBI) 2146
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)

Protocols

Product Specific Protocols
FC protocol for CL488 EZH2 antibody CL488-21800Download protocol
Standard Protocols
Click here to view our Standard Protocols