Various lysates were subjected to SDS PAGE followed by western blot with 85790-4-RR (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 85790-4-RR (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 85790-4-RR
IP result of anti-Ezrin (IP:85790-4-RR, 4ug; Detection:85790-4-RR 1:3000) with HeLa cells lysate 1280 ug.
IP result of anti-Ezrin (IP:85790-4-RR, 4ug; Detection:85790-4-RR 1:3000) with HeLa cells lysate 1280 ug.
IHC staining of Mouse small intestine using 85790-4-RR
Immunohistochemical analysis of paraffin-embedded Mouse small intestine tissue slide using 85790-4-RR (Ezrin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH6).
Immunohistochemical analysis of paraffin-embedded Mouse small intestine tissue slide using 85790-4-RR (Ezrin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH6).
IHC staining of mouse stomach using 85790-4-RR
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 85790-4-RR (Ezrin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 85790-4-RR (Ezrin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
IF Staining of HeLa using 85790-4-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ezrin antibody (85790-4-RR, Clone: 250065E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ezrin antibody (85790-4-RR, Clone: 250065E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Affinity and Kinetic Characterization of 85790-4-RR
Biolayer interferometry (BLl) kinetic assays of 85790-4-RR against Human Ezrin were performed. The affinity constant is 0.426 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse stomach tissue, Mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
85790-4-RR targets Ezrin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Ezrin is a member of the ERM (Ezrin, Radixin, Moesin) protein family and serves as a crucial linker between the cell membrane and the actin cytoskeleton. It plays significant roles in various cellular processes, including maintaining cell morphology, facilitating cell movement and adhesion, regulating signal transduction, and influencing apoptosis.
Various lysates were subjected to SDS PAGE followed by western blot with 85790-4-RR (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of Mouse small intestine using 85790-4-RR
Immunohistochemical analysis of paraffin-embedded Mouse small intestine tissue slide using 85790-4-RR (Ezrin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH6).
IHC staining of mouse stomach using 85790-4-RR
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 85790-4-RR (Ezrin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
IP Figures
IP experiment of HeLa using 85790-4-RR
IP result of anti-Ezrin (IP:85790-4-RR, 4ug; Detection:85790-4-RR 1:3000) with HeLa cells lysate 1280 ug.
IF/ICC Figures
IF Staining of HeLa using 85790-4-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ezrin antibody (85790-4-RR, Clone: 250065E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
AFFINITY Figures
Affinity and Kinetic Characterization of 85790-4-RR
Biolayer interferometry (BLl) kinetic assays of 85790-4-RR against Human Ezrin were performed. The affinity constant is 0.426 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Ezrin Recombinant antibody
Catalog Number
85790-4-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:2000-1:8000 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
