Ezrin Recombinant antibody

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  WB analysis using 85790-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85790-4-RR (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85790-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85790-4-RR against Human Ezrin were performed. The affinity constant is 0.426 nM.

Ezrin Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85790-4-RR

Clone No.250065E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EZR, VIL2, Villin-2

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Tested Applications

• Positive WB detected in: HeLa cells, Calu-3 cells, A431 cells, Jurkat cells, MOLT-4 cells, NIH/3T3 cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85790-4-RR targets Ezrin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Ezrin fusion protein Ag23530
• Full Name: ezrin
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC013903
• Gene Symbol: Ezrin
• Gene ID (NCBI): 7430
• ENSEMBL Gene ID: ENSG00000092820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15311
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ezrin is a member of the ERM (Ezrin, Radixin, Moesin) protein family and serves as a crucial linker between the cell membrane and the actin cytoskeleton. It plays significant roles in various cellular processes, including maintaining cell morphology, facilitating cell movement and adhesion, regulating signal transduction, and influencing apoptosis.