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  • KD/KO Validated

Factor XII Polyclonal antibody

Factor XII Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12551-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Coagulation factor XII, F12, Factor XII, HAE3, HAEX, HAF, Hageman factor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells
Positive IHC detected inhuman liver tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12551-1-AP targets Factor XII in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3401

Product name:

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: of

Sequence:

Predict reactive species
Full Name coagulation factor XII (Hageman factor)
Calculated Molecular Weight 615 aa, 68 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC012390
Gene Symbol Factor XII
Gene ID (NCBI) 2161
RRIDAB_1080025
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP00748
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.

Protocols

Product Specific Protocols
WB protocol for Factor XII antibody 12551-1-APDownload protocol
IHC protocol for Factor XII antibody 12551-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Commun

Blood coagulation factor XII drives adaptive immunity during neuroinflammation via CD87-mediated modulation of dendritic cells.

Authors - Kerstin Göbel
humanIHC

Thyroid

Clinical and Molecular Characterizations of Papillary Thyroid Cancer in Children and Young Adults: a Multicenter Retrospective Study.

Authors - Kai Guo
mouseWB,IHC

Int J Mol Med

FXII regulates the formation of deep vein thrombosis via the PI3K/AKT signaling pathway in mice.

Authors - Yan Meng
  • KD Validated
humanIHC

Breast Cancer (Dove Med Press)

Identification and Validation of Novel Metastasis-Related Immune Gene Signature in Breast Cancer

Authors - Shen Ma
pigWB

Vet Res

TRIM28 regulates the coagulation cascade inhibited by p72 of African swine fever virus

Authors - Xuejiao Zhu
  • KD Validated
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