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F2R Polyclonal antibody

F2R Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 26366-1-AP

Synonyms

CF2R, Coagulation factor II receptor, F2R, HTR, PAR 1, PAR1, Thrombin receptor, TR



Tested Applications

Positive WB detected inY79 cells, K-562 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26366-1-AP is F2R Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen F2R fusion protein Ag24378
Full Name coagulation factor II (thrombin) receptor
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC051909
Gene symbol F2R
Gene ID (NCBI) 2149
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protease-activated receptor-1 (PAR1) is a G protein coupled receptor.PAR1 has a greater affinity for thrombin and mediates rapid Ca2+ mobilization and platelet activation. PAR1 has a calculated molecular weight of 47 kDa, but can be detected as 70 kDa after posttranslational modification.(PMID: 26822533)

Protocols

Product Specific Protocols
WB protocol for F2R antibody 26366-1-APDownload protocol
IHC protocol for F2R antibody 26366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols