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F2R Polyclonal antibody

F2R Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 26366-1-AP


CF2R, Coagulation factor II receptor, F2R, HTR, PAR 1, PAR1, Thrombin receptor, TR

Tested Applications

Positive WB detected inY79 cells, K-562 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26366-1-AP targets F2R in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen F2R fusion protein Ag24378
Full Name coagulation factor II (thrombin) receptor
Calculated Molecular Weight 47 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC051909
Gene Symbol F2R
Gene ID (NCBI) 2149
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protease-activated receptor-1 (PAR1) is a G protein coupled receptor.PAR1 has a greater affinity for thrombin and mediates rapid Ca2+ mobilization and platelet activation. PAR1 has a calculated molecular weight of 47 kDa, but can be detected as 70 kDa after posttranslational modification.(PMID: 26822533)


Product Specific Protocols
WB protocol for F2R antibody 26366-1-APDownload protocol
IHC protocol for F2R antibody 26366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Breast Cancer Res Treat

Exosomal MMP-1 transfers metastasis potential in triple-negative breast cancer through PAR1-mediated EMT.

Authors - Yihui Zhu


Thrombin receptor activating peptide-6 decreases acute graft-versus-host disease through activating GPR15

Authors - Cong Liu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Sammy (Verified Customer) (02-15-2024)

Several unspecific bands detected by WB one of which was the correct size. IF staining worked well but caveated by potential lack of specificity.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1 in 1000 WB 1 in 200 IF
  • Cell Tissue Type: HUVEC lysate