F9 Polyclonal antibody

• Featured Product
• KD/KO Validated

F9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 21481-1-AP

Synonyms

Christmas factor, coagulation factor IX, F9, FIX, HEMB, PTC

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Validation Data Gallery

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  WB analysis of HepG2 using 21481-1-AP

  Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with 21481-1-AP (F9 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IHC staining of human liver cancer using 21481-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21481-1-AP (F9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 21481-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21481-1-AP (F9 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: Tunicamycin treated HepG2 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 21481-1-AP is F9 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: F9 fusion protein Ag15723
• Full Name: coagulation factor IX
• Calculated Molecular Weight: 461 aa, 52 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC109215
• Gene Symbol: F9
• Gene ID (NCBI): 2158
• RRID: AB_2878866
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The F9 gene encodes coagulation factor IX, which circulates as an inactive zymogen until proteolytic release of its activation peptide allows it to assume the conformation of an active serine protease. Its role in the blood coagulation cascade is to activate factor X (F10) through interactions with calcium, membrane phospholipids, and factor VIII (F8). Factor IX and factor X both consist of 2 polypeptide chains referred to as the L (light) and H (heavy) chains. The H chain bears a structural resemblance to the polypeptide chain of the pancreatic serine protease trypsin (PRSS1). The L chain is covalently linked to the H chain by a single disulfide bond.

Publications

Species	Application	Title
mouse	WB	EMBO Mol Med; CRISPR/Cas9-mediated somatic correction of a novel coagulator factor IX gene mutation ameliorates hemophilia in mouse.; Authors - Yuting Guan
human	WB	Front Neurosci; Serum Exosomal Proteins F9 and TSP-1 as Potential Diagnostic Biomarkers for Newly Diagnosed Epilepsy.; Authors - Zijun Lin
human	IF	Cell Death Dis; Genome wide CRISPR/Cas9 screen identifies the coagulation factor IX (F9) as a regulator of senescence.; Authors - Paula Carpintero-Fernández ; KD Validated