FABP1 Polyclonal antibody

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  WB analysis using 34024-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 34024-1-AP (FABP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse colon using 34024-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using FABP1 antibody (34024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse colon using 34024-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using FABP1 antibody (34024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat colon using 34024-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using FABP1 antibody (34024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FABP1 Polyclonal Antibody for WB, IF-P, ELISA

Cat No. 34024-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, pig

Applications

WB, IF-P, ELISA

Synonyms

14 kDa selenium-binding protein, Fabpl, Fatty acid-binding protein 1, Fatty acid-binding protein, liver, L-FABP

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat colon tissue, rat liver tissue, pig liver tissue
• Positive IF-P detected in: mouse colon tissue, rat colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

34024-1-AP targets FABP1 in WB, IF-P, ELISA applications and shows reactivity with mouse, rat, pig samples.

• Tested Reactivity: mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg7277

  Product name: Recombinant rat FABP1 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 2-127 aa of NM_012556.2

  Sequence: NFSGKYQVQSQENFEPFMKAMGLPEDLIQKGKDIKGVSEIVHEGKKVKLTITYGSKVIHNEFTLGEECELETMTGEKVKAVVKMEGDNKMVTTFKGIKSVTEFNGDTITNTMTLGDIVYKRVSKRI

• Full Name: fatty acid binding protein 1, liver
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: NM_012556.2
• Gene Symbol: Fabp1
• Gene ID (NCBI): 24360
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P02692
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FABP1, liver fatty acid-binding protein, is abundant in cytoplasm that regulates lipid transport and metabolism. It plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PMID:25732850). FABP1 is mainly expressed in hepatocytes, enterocytes and to a lesser degree in renal tubular cells, associated with liver injury (PMID: 15653098). Levels of FABP1 have been found to be elevated in patients with hepatocyte injury secondary to alcohol or drug toxicity (PMID: 14563446).