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FAK Antibody 9 Publications

Mouse Monoclonal| Catalog number: 66258-1-Ig |CloneNo.: 2C5B9

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Con: 214 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

FAK Antibody WB MCF-7 cells 66258-1-Ig

WB result of FAK antibody (66258-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAK transfected MCF-7 cells

FAK Antibody WB MCF-7 cells 66258-1-Ig

Western blot analysis of FAK-specific in various cell lines using Proteintech antibody 66258-1-Ig at dilution of 1:3000 incubated at room temperature for 1.5 hours

FAK Antibody IHC human breast cancer tissue 66258-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66258-1-Ig (FAK antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

FAK Antibody IHC human breast cancer tissue 66258-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66258-1-Ig (FAK antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

FAK Antibody IF A549 cells 66258-1-Ig

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed A549 cells using 66258-1-Ig(FAK antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
MCF-7 cells, human testis tissue, HepG2 cells, NIH/3T3 cells, RAW 264.7 cells, ROS1728 cells
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
A549 cells, HeLa cells
Recommended dilution:
WB : 1:1000-1:6000
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2c
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
FAK fusion protein Ag17966
Full name:
PTK2 protein tyrosine kinase 2
Calculated molecular weight:
1052aa,119 kDa
Observed molecular weight:
110 kDa
GenBank accession number:
BC028733
Gene ID (NCBI):
5747
Gene symbol
PTK2
Synonyms
FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2

Background

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.


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