• Featured Product
  • KD/KO Validated

FAK Antibody

FAK Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2C5B9

Cat no : 66258-1-Ig

Synonyms

FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2



Tested Applications

Positive WB detected inMCF-7 cells, human testis tissue, HepG2 cells, NIH/3T3 cells, RAW 264.7 cells, ROS1728 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66258-1-Ig targets FAK in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen FAK fusion protein Ag17966
Full Name PTK2 protein tyrosine kinase 2
Calculated molecular weight 1052aa,119 kDa
Observed molecular weight 110 kDa
GenBank accession number BC028733
Gene symbol PTK2
Gene ID (NCBI) 5747
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.

Protocols

Product Specific Protocols
WB protocol for FAK antibody 66258-1-IgDownload protocol
IHC protocol for FAK antibody 66258-1-IgDownload protocol
IF protocol for FAK antibody 66258-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Inflammation

TNF-α Regulates ITGβ1 and SYND4 Expression in Nucleus Pulposus Cells: Activation of FAK/PI3K Signaling.

Authors - Xinghuo Wu
humanIF

Cells

Subcutaneous and Visceral Adipose-Derived Mesenchymal Stem Cells: Commonality and Diversity.

Authors - Andreas Ritter
humanWB

Mol Med Rep

RSRC1 suppresses gastric cancer cell proliferation and migration by regulating PTEN expression.

Authors - Shijun Yu
humanWB

Biol Open

Quantitative proteomic profiling of tumor-associated vascular endothelial cells in colorectal cancer.

Authors - Guoqiang Wang
humanWB

IUBMB Life

Downregulation of STRAP promotes tumor growth and metastasis in hepatocellular carcinoma via reducing PTEN level.

Authors - Qiong Wu
humanWB

J Cell Biochem

DHPAC, a novel microtubule depolymerizing agent, suppresses angiogenesis and vasculogenic mimicry formation of human non-small cell lung cancer.

Authors - Fu-Lian Gong