Validation Data Gallery
|Positive WB detected in||MCF-7 cells, human testis tissue, HepG2 cells, NIH/3T3 cells, RAW 264.7 cells, ROS1728 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HUVEC cells, HeLa cells, A549 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66258-1-Ig targets FAK in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2c|
|Immunogen||FAK fusion protein Ag17966|
|Full Name||PTK2 protein tyrosine kinase 2|
|Calculated molecular weight||1052 aa, 119 kDa|
|Observed molecular weight||110 kDa|
|GenBank accession number||BC028733|
|Gene ID (NCBI)||5747|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.
TNF-α Regulates ITGβ1 and SYND4 Expression in Nucleus Pulposus Cells: Activation of FAK/PI3K Signaling.
Subcutaneous and Visceral Adipose-Derived Mesenchymal Stem Cells: Commonality and Diversity.
Cell Death Dis
Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src.
Rab34 regulates adhesion, migration, and invasion of breast cancer cells.
Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.
Aging (Albany NY)
TNFSF9 promotes metastasis of pancreatic cancer through Wnt/Snail signaling and M2 polarization of macrophages.