FAK Monoclonal antibody

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• KD/KO Validated

FAK Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

25

CloneNo.

2C5B9

Cat no : 66258-1-Ig

Synonyms

FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2

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Validation Data Gallery

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  WB analysis of MCF-7 using 66258-1-Ig

  WB result of FAK antibody (66258-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAK transfected MCF-7 cells.

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  WB analysis of MCF-7 using 66258-1-Ig

  Western blot analysis of FAK-specific in various cell lines using Proteintech antibody 66258-1-Ig at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66258-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66258-1-Ig (FAK Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66258-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66258-1-Ig (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 66258-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66258-1-Ig (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66258-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66258-1-Ig (FAK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66258-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66258-1-Ig (FAK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 66258-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using FAK antibody (66258-1-Ig, Clone: 2C5B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of A549 using 66258-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using FAK antibody (66258-1-Ig, Clone: 2C5B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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  IF Staining of HeLa using 66258-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66258-1-Ig (FAK antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, human testis tissue, HepG2 cells, NIH/3T3 cells, RAW 264.7 cells, ROS1728 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HUVEC cells, HeLa cells, A549 cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®555 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 22 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

66258-1-Ig targets FAK in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2c
• Class: Monoclonal
• Type: Antibody
• Immunogen: FAK fusion protein Ag17966
• Full Name: PTK2 protein tyrosine kinase 2
• Calculated molecular weight: 1052 aa, 119 kDa
• Observed molecular weight: 110 kDa
• GenBank accession number: BC028733
• Gene symbol: PTK2
• Gene ID (NCBI): 5747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.

Publications

Species	Application	Title
	WB	Inflammation; TNF-α Regulates ITGβ1 and SYND4 Expression in Nucleus Pulposus Cells: Activation of FAK/PI3K Signaling.; Authors - Xinghuo Wu
human	IF	Cells; Subcutaneous and Visceral Adipose-Derived Mesenchymal Stem Cells: Commonality and Diversity.; Authors - Andreas Ritter
human	WB	Cell Death Dis; Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src.; Authors - Huiying Liu
human	WB	Oncogene; Rab34 regulates adhesion, migration, and invasion of breast cancer cells.; Authors - Lixiang Sun
rat	WB	Acta Biomater; Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.; Authors - Shanshan Ma
human	WB	Aging (Albany NY); TNFSF9 promotes metastasis of pancreatic cancer through Wnt/Snail signaling and M2 polarization of macrophages.; Authors - Jiao Wu