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- KD/KO Validated
FAK Monoclonal antibody
FAK Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2c
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2C5B9
Cat no : 66258-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, human testis tissue, HepG2 cells, NIH/3T3 cells, RAW 264.7 cells, ROS1728 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HUVEC cells, HeLa cells, A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 22 publications below |
IF | See 5 publications below |
IP | See 1 publications below |
Product Information
66258-1-Ig targets FAK in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2c |
Class | Monoclonal |
Type | Antibody |
Immunogen | FAK fusion protein Ag17966 |
Full Name | PTK2 protein tyrosine kinase 2 |
Calculated molecular weight | 1052 aa, 119 kDa |
Observed molecular weight | 110 kDa |
GenBank accession number | BC028733 |
Gene symbol | PTK2 |
Gene ID (NCBI) | 5747 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for FAK antibody 66258-1-Ig | Download protocol |
IHC protocol for FAK antibody 66258-1-Ig | Download protocol |
IF protocol for FAK antibody 66258-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Inflammation TNF-α Regulates ITGβ1 and SYND4 Expression in Nucleus Pulposus Cells: Activation of FAK/PI3K Signaling. | ||
Cells Subcutaneous and Visceral Adipose-Derived Mesenchymal Stem Cells: Commonality and Diversity. | ||
Cell Death Dis Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src. | ||
Acta Biomater Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury. | ||
Aging (Albany NY) TNFSF9 promotes metastasis of pancreatic cancer through Wnt/Snail signaling and M2 polarization of macrophages. |