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CoraLite® Plus 488-conjugated FAK Monoclonal antibody

FAK Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2c

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C5B9

Cat no : CL488-66258

Synonyms

FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2



Tested Applications

Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66258 targets FAK in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen FAK fusion protein Ag17966
Full Name PTK2 protein tyrosine kinase 2
Calculated Molecular Weight 1052 aa, 119 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC028733
Gene Symbol PTK2
Gene ID (NCBI) 5747
RRIDAB_2919306
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 FAK antibody CL488-66258Download protocol
Standard Protocols
Click here to view our Standard Protocols