FAM114A2 Polyclonal antibody

• 

  WB analysis using 29917-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29917-1-AP (FAM114A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 29917-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FAM114A2 antibody (29917-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FAM114A2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 29917-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF/ICC, ELISA

Synonyms

133K02, C5orf3, FAM114A2, Protein FAM114A2

---

Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells, MOLT-4 cells, U-251 cells, mouse testis tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29917-1-AP targets FAM114A2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag31963

  Product name: Recombinant human FAM114A2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 255-505 aa of BC011414

  Sequence: MLSQESEIKVKSILNSLSGEELETLKVELEQLKETFSLAEFCEEEEEEKKGDEDFTKDITELFSQLHVSSKPEKLARARNTAHEWIRKSLTKPLAENEEGEKQSEAENTEQVNKNSIEDIHAFAIRSLAELTACSIELFHKTAALVLHGRKQEVTAIERSQTLSQMTIVLCKELSSLSKEFTTCLTTAGVKEMADVLNPLITAVFLEASNSASYIQDAFQLLLPVLEISLIENKIESHRHELQGQKPLLEH

• Full Name: family with sequence similarity 114, member A2
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC011414
• Gene Symbol: FAM114A2
• Gene ID (NCBI): 10827
• RRID: AB_3086188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NRY5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM114A2, also known as C5orf3, belongs to the nervous overexpress protein family and has been implicated in neuronal cell development. FAM114A2 a human testis enrich gene and testis sperm-binding protein, is highly conserved in mammals. The observed band size is around 55 kDa.