FAM134C Polyclonal antibody

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  WB analysis using 26330-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26330-1-AP (FAM134C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 26330-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using FAM134C antibody (26330-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

FAM134C Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 26330-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RETREG3, Reticulophagy regulator 3

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, HuH-7 cellls
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26330-1-AP targets FAM134C in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM134C fusion protein Ag23680
• Full Name: family with sequence similarity 134, member C
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC049370
• Gene Symbol: FAM134C
• Gene ID (NCBI): 162427
• RRID: AB_3085859
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86VR2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein family with sequence similarity 134 (FAM134) comprises three proteins: FAM134B, FAM134A, and FAM134C. These proteins share the same LIR amino acid sequence. FAM134C, also known as the reticulophagy regulator 3 (RETREG3), plays a crucial role as an ER-phagy receptor. It is essential for maintaining ER morphology in a LC3-interacting region (LIR)-dependent manner.