Tested Applications
| Positive WB detected in | A549 cells |
| Positive IP detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27738-1-AP targets FAM91A1 in WB, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26902 Product name: Recombinant human FAM91A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-91 aa of BC030520 Sequence: MNIDVEFHIRHNYPWNKLPANVRQSLGNSQREYEKQVVLYSIRNQLRYRNNLVKHVKKDERRYYEELLKYSRDHLMLYPYHLSDIVCYVCL Predict reactive species |
| Full Name | family with sequence similarity 91, member A1 |
| Observed Molecular Weight | 94 kDa |
| GenBank Accession Number | BC030520 |
| Gene Symbol | FAM91A1 |
| Gene ID (NCBI) | 157769 |
| RRID | AB_2880957 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q658Y4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for FAM91A1 antibody 27738-1-AP | Download protocol |
| WB protocol for FAM91A1 antibody 27738-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FAM91A1 Polyclonal antibody (Cat# 27738-1-AP) has 5 citations published in Cell, Cell Discovery, Structure, bioRxiv, and Molecular Cell. Research fields span intracellular trafficking (WDR11 complex, endosome-to-TGN transport), innate immune signaling (STING-IFN-I pathway), structural biology (cryo-EM of FAM91A1-containing complexes), gene therapy (rAAV transduction), and DNA repair (homologous recombination). Applications cited include WB, IP, and CoIP in human and mouse systems.
| Species | Application | Title |
|---|---|---|
Cell Discov Trans-Golgi network tethering factors regulate TBK1 trafficking and promote the STING-IFN-I pathway | ||
Structure Cryo-EM structure of the WDR11-FAM91A1-C17orf75 complex involved in retrograde trafficking. | ||
bioRxiv TBC1D23-AAVR Interaction Drives Endosome-to-TGN Trafficking Required for rAAV Transduction. | ||
Reviews
What customers say
The single review for this antibody (catalog 27738-1-AP) is a 5-star rating from a Western Blot application. Using a 1:1,000 dilution on HEK293T cells, the reviewer reported a very strong main band appearing between the 85 and 118 kDa markers, consistent with the expected ~94 kDa size of FAM91A1. Overall, the feedback is positive, highlighting strong and specific detection at the anticipated molecular weight.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Christine (Verified Customer) (01-27-2023) | Detects a very strong main band between the 85 and 118 kDa markers (expected size of FAM91A1 is 94 kDa)
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