FANCD2 Polyclonal antibody

FANCD2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 24006-1-AP

Synonyms

FA D2, FA4, FACD, FAD, FAD2, FANCD, FANCD2, Protein FACD2



Tested Applications

Positive WB detected inHL-60 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

24006-1-AP targets FANCD2 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Fanconi anemia, complementation group D2
Calculated molecular weight 166 kDa
Observed molecular weight 150-155 kDa
GenBank accession numberNM_033084
Gene symbol FANCD2
Gene ID (NCBI) 2177
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FANCD2, also named as FACD and FACD2, is required for maintenance of chromosomal stability. FANCD2 promotes accurate and efficient pairing of homologs during meiosis. FANCD2 is involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. It may participate in S phase and G2 phase checkpoint activation upon DNA damage. It promotes BRCA2/FANCD1 loading onto damaged chromatin. FANCD2 may also be involved in B-cell immunoglobulin isotype switching. Defects in FANCD2 are a cause of Fanconi anemia (FA) which is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. The antibody recognize both un-phospho and phospho-Ser(330) of FANCD2.

Protocols

Product Specific Protocols
WB protocol for FANCD2 antibody 24006-1-APDownload protocol
IHC protocol for FANCD2 antibody 24006-1-APDownload protocol
IP protocol for FANCD2 antibody 24006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FEBS Open Bio

Augmentation of the therapeutic efficacy of WEE1 kinase inhibitor AZD1775 by inhibiting the YAP-E2F1-DNA damage response pathway axis.

Authors - Yusuke Oku