FANCD2 Polyclonal antibody

FANCD2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 24006-1-AP

Synonyms

FA D2, FA4, FACD, FAD, FAD2, FANCD, FANCD2, Protein FACD2

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Validation Data Gallery

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  WB analysis of HL-60 using 24006-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 24006-1-AP (FANCD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 24006-1-AP

  IP result of anti-FANCD2 (IP:24006-1-AP, 4ug; Detection:24006-1-AP 1:300) with mouse testis tissue lysate 6000ug.

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  IHC staining of human testis using 24006-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 24006-1-AP (FANCD2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 24006-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 24006-1-AP (FANCD2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HL-60 cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

24006-1-AP targets FANCD2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Fanconi anemia, complementation group D2
• Calculated Molecular Weight: 166 kDa
• Observed Molecular Weight: 150-155 kDa
• GenBank Accession Number: NM_033084
• Gene Symbol: FANCD2
• Gene ID (NCBI): 2177
• RRID: AB_11232601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FANCD2, also named as FACD and FACD2, is required for maintenance of chromosomal stability. FANCD2 promotes accurate and efficient pairing of homologs during meiosis. FANCD2 is involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. It may participate in S phase and G2 phase checkpoint activation upon DNA damage. It promotes BRCA2/FANCD1 loading onto damaged chromatin. FANCD2 may also be involved in B-cell immunoglobulin isotype switching. Defects in FANCD2 are a cause of Fanconi anemia (FA) which is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. The antibody recognize both un-phospho and phospho-Ser(330) of FANCD2.

Publications

Species	Application	Title
human	WB,IHC	Apoptosis; Crosstalk of ferroptosis regulators and tumor immunity in pancreatic adenocarcinoma: novel perspective to mRNA vaccines and personalized immunotherapy; Authors - Yanlong Shi
human	IHC	BMC Cancer; Comprehensive analysis of the autophagy-dependent ferroptosis-related gene FANCD2 in lung adenocarcinoma.; Authors - Huikai Miao
human	WB	FEBS Open Bio; Augmentation of the therapeutic efficacy of WEE1 kinase inhibitor AZD1775 by inhibiting the YAP-E2F1-DNA damage response pathway axis.; Authors - Yusuke Oku
human	WB,IF	Cell Death Differ; Oxoglutarate dehydrogenase-like inhibits the progression of hepatocellular carcinoma by inducing DNA damage through non-canonical function; Authors - Xiang Jiang
human	WB	Commun Biol; Fanconi anemia associated protein 20 (FAAP20) plays an essential role in homology-directed repair of DNA double-strand breaks; Authors - Anna Palovcak