FASTKD2 Antibody

• Featured Product
• KD/KO Validated

FASTKD2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 17464-1-AP

Synonyms

FAST kinase domains 2, FASTKD2, KIAA0971

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Validation Data Gallery

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  WB analysis of mouse heart tissue using 17464-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17464-1-AP (FASTKD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF staining of COS7 cells using 17464-1-AP

  IF result of anti-FASTKD2 (17464-1-AP) with COS7 cells.

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  IP experiment of mouse heart tissue using 17464-1-AP

  IP Result of anti-FASTKD2 (IP:17464-1-AP, 4ug; Detection:17464-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse heart tissue, transfected cells, mouse brain tissue
• Positive IP detected in: mouse heart tissue
• Positive IF detected in: COS7 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Product Information

17464-1-AP targets FASTKD2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FASTKD2 fusion protein Ag11314
• Full Name: FAST kinase domains 2
• Calculated molecular weight: 710 aa, 81 kDa
• Observed molecular weight: 73-75 kDa
• GenBank accession number: BC001544
• Gene symbol: FASTKD2
• Gene ID (NCBI): 22868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FASTKD2(FAST kinase domain-containing protein 2) is also named as KIAA0971 and belongs to the FAST kinase family.A potential cleavage site of the 79 kDa FASTKD2 precursor protein by the mitochondrial matrix protease (MMP) is present between M52 and Q53 and predicts the mature imported protein from the FASTKD2 precursor to be composed of 642 amino acid residues for a MW of approximately 73 kDa(PMID: 18771761) and the clinical and MRI findings of the FASTKD2-associated disease are rather unusual for mitochondrial disorders.

Publications

Species	Application	Title
human	WB,IHC	J Cell Biochem; FASTKD2 promotes cancer cell progression through upregulating Myc expression in pancreatic ductal adenocarcinoma.; Authors - Rui Fang ; KD Validated
human	WB	J Biol Chem; Role of FAST kinase domains 3 (FASTKD3) in post-transcriptional regulation of mitochondrial gene expression.; Authors - Erik Boehm
	IF	J Biol Chem; The Pseudouridine Synthase RPUSD4 is an essential component of Mitochondrial RNA Granules.; Authors - Sofia Zaganelli
human,zebrafish	WB	Hum Mutat; Mutations in FASTKD2 are associated with mitochondrial disease with multi-OXPHOS deficiency.; Authors - Xiujuan Wei ; KD Validated
		J Cell Sci; Mitochondrial Alkbh1 localises to mtRNA granules and its knockdown induces mitochondrial UPR in humans and C. elegans.; Authors - Anita Wagner
	WB	RNA; FASTKD2 is an RNA-binding protein required for mitochondrial RNA processing and translation.; Authors - Johannes Popow ; KD Validated