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  • KD/KO Validated

FASTKD2 Polyclonal antibody

FASTKD2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17464-1-AP

Synonyms

FAST kinase domains 2, FASTKD2, KIAA0971



Tested Applications

Positive WB detected inmouse heart tissue
Positive IP detected inmouse heart tissue
Positive IF detected inCOS7 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17464-1-AP targets FASTKD2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FASTKD2 fusion protein Ag11314
Full Name FAST kinase domains 2
Calculated molecular weight 710 aa, 81 kDa
Observed molecular weight 73-75 kDa
GenBank accession numberBC001544
Gene symbol FASTKD2
Gene ID (NCBI) 22868
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715)

Protocols

Product Specific Protocols
WB protocol for FASTKD2 antibody 17464-1-APDownload protocol
IF protocol for FASTKD2 antibody 17464-1-APDownload protocol
IP protocol for FASTKD2 antibody 17464-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Cell Biochem

FASTKD2 promotes cancer cell progression through upregulating Myc expression in pancreatic ductal adenocarcinoma.

Authors - Rui Fang
  • KD Validated
humanWB

J Biol Chem

Role of FAST kinase domains 3 (FASTKD3) in post-transcriptional regulation of mitochondrial gene expression.

Authors - Erik Boehm
IF

J Biol Chem

The Pseudouridine Synthase RPUSD4 is an essential component of Mitochondrial RNA Granules.

Authors - Sofia Zaganelli
human,zebrafishWB

Hum Mutat

Mutations in FASTKD2 are associated with mitochondrial disease with multi-OXPHOS deficiency.

Authors - Xiujuan Wei
  • KD Validated

J Cell Sci

Mitochondrial Alkbh1 localises to mtRNA granules and its knockdown induces mitochondrial UPR in humans and C. elegans.

Authors - Anita Wagner
WB

RNA

FASTKD2 is an RNA-binding protein required for mitochondrial RNA processing and translation.

Authors - Johannes Popow
  • KD Validated