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  • KD/KO Validated

FASTKD2 Antibody

FASTKD2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17464-1-AP

Synonyms

FAST kinase domains 2, FASTKD2, KIAA0971



Tested Applications

Positive WB detected inmouse heart tissue, transfected cells, mouse brain tissue
Positive IP detected inmouse heart tissue
Positive IF detected inCOS7 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17464-1-AP targets FASTKD2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FASTKD2 fusion protein Ag11314
Full Name FAST kinase domains 2
Calculated molecular weight 710 aa, 81 kDa
Observed molecular weight 73-75 kDa
GenBank accession numberBC001544
Gene symbol FASTKD2
Gene ID (NCBI) 22868
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FASTKD2(FAST kinase domain-containing protein 2) is also named as KIAA0971 and belongs to the FAST kinase family.A potential cleavage site of the 79 kDa FASTKD2 precursor protein by the mitochondrial matrix protease (MMP) is present between M52 and Q53 and predicts the mature imported protein from the FASTKD2 precursor to be composed of 642 amino acid residues for a MW of approximately 73 kDa(PMID: 18771761) and the clinical and MRI findings of the FASTKD2-associated disease are rather unusual for mitochondrial disorders.

Protocols

Product Specific Protocols
WB protocol for FASTKD2 antibody 17464-1-APDownload protocol
IF protocol for FASTKD2 antibody 17464-1-APDownload protocol
IP protocol for FASTKD2 antibody 17464-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Cell Biochem

FASTKD2 promotes cancer cell progression through upregulating Myc expression in pancreatic ductal adenocarcinoma.

Authors - Rui Fang
  • KD Validated
humanWB

J Biol Chem

Role of FAST kinase domains 3 (FASTKD3) in post-transcriptional regulation of mitochondrial gene expression.

Authors - Erik Boehm
IF

J Biol Chem

The Pseudouridine Synthase RPUSD4 is an essential component of Mitochondrial RNA Granules.

Authors - Sofia Zaganelli
human,zebrafishWB

Hum Mutat

Mutations in FASTKD2 are associated with mitochondrial disease with multi-OXPHOS deficiency.

Authors - Xiujuan Wei
  • KD Validated

J Cell Sci

Mitochondrial Alkbh1 localises to mtRNA granules and its knockdown induces mitochondrial UPR in humans and C. elegans.

Authors - Anita Wagner
WB

RNA

FASTKD2 is an RNA-binding protein required for mitochondrial RNA processing and translation.

Authors - Johannes Popow
  • KD Validated