Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | COS7 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17464-1-AP targets FASTKD2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11314 Product name: Recombinant human FASTKD2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 361-710 aa of BC001544 Sequence: ILLDECSKVVLDNIHGCPLRIMINILQSCKDLQYHNLDLFKGLADYVAATFDIWKFRKVLFILILFENLGFRPVGLMDLFMKRIVEDPESLNMKNILSILHTYSSLNHVYKCQNKEQFVEVMASALTGYLHTISSENLLDAVYSFCLMNYFPLAPFNQLLQKDIISELLTSDDMKNAYKLHTLDTCLKLDDTVYLRDIALSLPQLPRELPSSHTNAKVAEVLSSLLGGEGHFSKDVHLPHNYHIDFEIRMDTNRNQVLPLSDVDTTSATDIQRVAVLCVSRSAYCLGSSHPRGFLAMKMRHLNAMGFHVILVNNWEMDKLEMEDAVTFLKTKIYSVEALPVAAVNVQSTQ Predict reactive species |
| Full Name | FAST kinase domains 2 |
| Calculated Molecular Weight | 710 aa, 81 kDa |
| Observed Molecular Weight | 55-65 kDa |
| GenBank Accession Number | BC001544 |
| Gene Symbol | FASTKD2 |
| Gene ID (NCBI) | 22868 |
| RRID | AB_2101119 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NYY8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FASTKD2 antibody 17464-1-AP | Download protocol |
| IP protocol for FASTKD2 antibody 17464-1-AP | Download protocol |
| WB protocol for FASTKD2 antibody 17464-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics. | ||
Cell Metab A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation. | ||
Proc Natl Acad Sci U S A Cytosolic N6AMT1-dependent translation supports mitochondrial RNA processing | ||
Nucleic Acids Res FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain. | ||
EMBO Rep A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability. | ||
Cell Rep A mitochondria-specific isoform of FASTK is present in mitochondrial RNA granules and regulates gene expression and function. |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Erik (Verified Customer) (02-25-2021) | We used the antibody at 1:500 dilution, and it produced two clean bands for the two protein isoforms annotated in UniProt, at the expected height
 |
