Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | COS7 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17464-1-AP targets FASTKD2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11314 Product name: Recombinant human FASTKD2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 361-710 aa of BC001544 Sequence: ILLDECSKVVLDNIHGCPLRIMINILQSCKDLQYHNLDLFKGLADYVAATFDIWKFRKVLFILILFENLGFRPVGLMDLFMKRIVEDPESLNMKNILSILHTYSSLNHVYKCQNKEQFVEVMASALTGYLHTISSENLLDAVYSFCLMNYFPLAPFNQLLQKDIISELLTSDDMKNAYKLHTLDTCLKLDDTVYLRDIALSLPQLPRELPSSHTNAKVAEVLSSLLGGEGHFSKDVHLPHNYHIDFEIRMDTNRNQVLPLSDVDTTSATDIQRVAVLCVSRSAYCLGSSHPRGFLAMKMRHLNAMGFHVILVNNWEMDKLEMEDAVTFLKTKIYSVEALPVAAVNVQSTQ Predict reactive species |
| Full Name | FAST kinase domains 2 |
| Calculated Molecular Weight | 710 aa, 81 kDa |
| Observed Molecular Weight | 55-65 kDa |
| GenBank Accession Number | BC001544 |
| Gene Symbol | FASTKD2 |
| Gene ID (NCBI) | 22868 |
| RRID | AB_2101119 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NYY8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FASTKD2 antibody 17464-1-AP | Download protocol |
| IP protocol for FASTKD2 antibody 17464-1-AP | Download protocol |
| WB protocol for FASTKD2 antibody 17464-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FASTKD2 Polyclonal antibody (Cat# 17464-1-AP) has 24 citations published in journals including Nature Cell Biology, Cell Metabolism, PNAS, Nucleic Acids Research, Cell Reports, EMBO Reports, Journal of Biological Chemistry, and others. Research fields span mitochondrial RNA granules, posttranscriptional RNA processing, oxidative phosphorylation, phase separation, mitochondrial disease, and cancer biology (pancreatic adenocarcinoma, glioma). Applications cited include WB, IF, IHC, and RIP in human, mouse, and zebrafish samples.
| Species | Application | Title |
|---|---|---|
Cell Metab A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation. | ||
Nat Cell Biol Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics. | ||
Nucleic Acids Res mtFociCounter for automated single-cell mitochondrial nucleoid quantification and reproducible foci analysis | ||
Nucleic Acids Res Let's make it clear: systematic exploration of mitochondrial DNA- and RNA-protein complexes by complexome profiling | ||
Nucleic Acids Res FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain. | ||
Proc Natl Acad Sci U S A Cytosolic N6AMT1-dependent translation supports mitochondrial RNA processing |
Reviews
What customers say
The single review for 17464-1-AP is from Erik Dassi (February 2021), who rated it 4 out of 5 stars. He used the antibody at a 1:500 dilution for Western Blot on PANC1 and MIAPACA2 cell lines and reported that it produced two clean bands corresponding to the two protein isoforms annotated in UniProt, at the expected molecular weight. Overall, the reviewer found the antibody performed well with clear, specific results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Erik (Verified Customer) (02-25-2021) | We used the antibody at 1:500 dilution, and it produced two clean bands for the two protein isoforms annotated in UniProt, at the expected height
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