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FASTKD2 Polyclonal antibody
FASTKD2 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 17464-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells |
Positive IP detected in | mouse heart tissue |
Positive IF detected in | COS7 cells, U2OS cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 4 publications below |
WB | See 7 publications below |
IHC | See 1 publications below |
IF | See 5 publications below |
IP | See 2 publications below |
Product Information
17464-1-AP targets FASTKD2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FASTKD2 fusion protein Ag11314 |
Full Name | FAST kinase domains 2 |
Calculated molecular weight | 710 aa, 81 kDa |
Observed molecular weight | 55-65 kDa |
GenBank accession number | BC001544 |
Gene symbol | FASTKD2 |
Gene ID (NCBI) | 22868 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715)
Protocols
Product Specific Protocols | |
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WB protocol for FASTKD2 antibody 17464-1-AP | Download protocol |
IF protocol for FASTKD2 antibody 17464-1-AP | Download protocol |
IP protocol for FASTKD2 antibody 17464-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Cell Biochem FASTKD2 promotes cancer cell progression through upregulating Myc expression in pancreatic ductal adenocarcinoma.
| ||
J Biol Chem Role of FAST kinase domains 3 (FASTKD3) in post-transcriptional regulation of mitochondrial gene expression. | ||
J Biol Chem The Pseudouridine Synthase RPUSD4 is an essential component of Mitochondrial RNA Granules. | ||
Hum Mutat Mutations in FASTKD2 are associated with mitochondrial disease with multi-OXPHOS deficiency.
| ||
J Cell Sci Mitochondrial Alkbh1 localises to mtRNA granules and its knockdown induces mitochondrial UPR in humans and C. elegans. |