FASTKD2 Antibody 17464-1-AP

Tested Applications

Applications:

ELISA, IF, IP, WB
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mouse brain tissue were subjected to SDS PAGE followed by western blot with 17464-1-AP(FASTKD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IF result of anti-FASTKD2 (17464-1-AP) with COS7 cells.

IP Result of anti-FASTKD2 (IP:17464-1-AP, 4ug; Detection:17464-1-AP 1:300) with mouse heart tissue lysate 4000ug.

Species specificity:

human, mouse, rat

Positive WB detected in:

mouse brain tissue, mouse brain tissue, mouse heart tissue

Positive IP detected in:

mouse heart tissue

Positive IF detected in:

COS7 cells, U2OS cells

Recommended dilution:

WB : 1:500-1:2000

IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB

IF : 1:20-1:200

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

FASTKD2 fusion protein ag11314

Full name:

FAST kinase domains 2

Calculated molecular weight:

710aa,81 kDa

Observed molecular weight:

73-75 kDa

GenBank accession number:

BC001544

Gene ID (NCBI):

22868

Gene symbol

FASTKD2

Synonyms

FAST kinase domains 2, FASTKD2, KIAA0971

Background

FASTKD2(FAST kinase domain-containing protein 2) is also named as KIAA0971 and belongs to the FAST kinase family.A potential cleavage site of the 79 kDa FASTKD2 precursor protein by the mitochondrial matrix protease (MMP) is present between M52 and Q53 and predicts the mature imported protein from the FASTKD2 precursor to be composed of 642 amino acid residues for a MW of approximately 73 kDa(PMID: 18771761) and the clinical and MRI findings of the FASTKD2-associated disease are rather unusual for mitochondrial disorders.

Showing 1- [6] of [7] Publications

Author:		Johannes Popow
Pubmed ID:		26370583
Journal:		RNA
Title:		FASTKD2 is an RNA-binding protein required for mitochondrial RNA processing and translation.
Application:		WB
KD validated
Species:

Author:		Hana Antonicka
Pubmed ID:		25683715
Journal:		Cell Rep
Title:		Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.
Application:		WB, IF, IP, IP
KD validated
Species:		human

Author:		Alexis A Jourdain
Pubmed ID:		25704814
Journal:		Cell Rep
Title:		A mitochondria-specific isoform of FASTK is present in mitochondrial RNA granules and regulates gene expression and function.
Application:		IF

Species:

Author:		Erik Boehm
Pubmed ID:		27789713
Journal:		J Biol Chem
Title:		Role of FAST kinase domains 3 (FASTKD3) in post-transcriptional regulation of mitochondrial gene expression.
Application:		WB

Species:		human

Author:		Hana Antonicka
Pubmed ID:		27974379
Journal:		EMBO Rep
Title:		A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.
Application:		WB

Species:		human

Author:		Sofia Zaganelli
Pubmed ID:		28082677
Journal:		J Biol Chem
Title:		The Pseudouridine Synthase RPUSD4 is an essential component of Mitochondrial RNA Granules.
Application:		IF

Species:

WB Figures

mouse brain tissue were subjected to SDS PAGE followed by western blot with 17464-1-AP(FASTKD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 17464-1-AP(FASTKD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse heart tissue were subjected to SDS PAGE followed by western blot with 17464-1-AP(FASTKD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

mouse heart tissue were subjected to SDS PAGE followed by western blot with 17464-1-AP(FASTKD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

WB result of anti-FASTKD2 (17464-1-AP) with overexpressed protein.

IP Figures

IP Result of anti-FASTKD2 (IP:17464-1-AP, 4ug; Detection:17464-1-AP 1:300) with mouse heart tissue lysate 4000ug.

IF Figures

IF result of anti- FASTKD2 (17464-1-AP) with FASTKD2-YFP overexpression in COS7 cells.

IF result of anti-FASTKD2 (17464-1-AP) with COS7 cells.

IF result of anti-FASTKD2 (17464-1-AP) with U2OS cells.

FASTKD2 Antibody 7 Publications

Rabbit Polyclonal| Catalog number: 17464-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [6] of [7] Publications

WB Figures

IP Figures

IF Figures

FASTKD2 Antibody 7 Publications

Rabbit Polyclonal| Catalog number: 17464-1-AP

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Tested Applications

Product Information

Immunogen Information

Background