|Positive WB detected in||HeLa cells, HepG2 cells, K-562 cells|
|Positive IP detected in||mouse heart tissue|
|Positive IF detected in||COS7 cells, U2OS cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17464-1-AP targets FASTKD2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FASTKD2 fusion protein Ag11314|
|Full Name||FAST kinase domains 2|
|Calculated molecular weight||710 aa, 81 kDa|
|Observed molecular weight||55-65 kDa|
|GenBank accession number||BC001544|
|Gene ID (NCBI)||22868|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715)
Methods Mol Biol
Sucrose Gradient Sedimentation Analysis of Mitochondrial Ribosomes.
J Cell Biochem
FASTKD2 promotes cancer cell progression through upregulating Myc expression in pancreatic ductal adenocarcinoma.
J Biol Chem
Role of FAST kinase domains 3 (FASTKD3) in post-transcriptional regulation of mitochondrial gene expression.
J Biol Chem
The Pseudouridine Synthase RPUSD4 is an essential component of Mitochondrial RNA Granules.
Mutations in FASTKD2 are associated with mitochondrial disease with multi-OXPHOS deficiency.
J Cell Sci
Mitochondrial Alkbh1 localises to mtRNA granules and its knockdown induces mitochondrial UPR in humans and C. elegans.