FASTKD2 Polyclonal antibody

• Featured Product
• KD/KO Validated

FASTKD2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 17464-1-AP

Synonyms

FAST kinase domains 2, FASTKD2, KIAA0971

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Validation Data Gallery

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  WB analysis using 17464-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17464-1-AP (FASTKD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of COS7 cells using 17464-1-AP

  IF result of anti-FASTKD2 (17464-1-AP) with COS7 cells.

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  IF Staining of U2OS using 17464-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 17464-1-AP (FASTKD2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IP experiment of mouse heart using 17464-1-AP

  IP Result of anti-FASTKD2 (IP:17464-1-AP, 4ug; Detection:17464-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells
• Positive IP detected in: mouse heart tissue
• Positive IF detected in: COS7 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Product Information

17464-1-AP targets FASTKD2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FASTKD2 fusion protein Ag11314
• Full Name: FAST kinase domains 2
• Calculated molecular weight: 710 aa, 81 kDa
• Observed molecular weight: 55-65 kDa
• GenBank accession number: BC001544
• Gene symbol: FASTKD2
• Gene ID (NCBI): 22868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715)

Publications

Species	Application	Title
human	IF	Methods Mol Biol; Sucrose Gradient Sedimentation Analysis of Mitochondrial Ribosomes.; Authors - Austin Choi
human	WB,IHC	J Cell Biochem; FASTKD2 promotes cancer cell progression through upregulating Myc expression in pancreatic ductal adenocarcinoma.; Authors - Rui Fang ; KD Validated
human	WB	J Biol Chem; Role of FAST kinase domains 3 (FASTKD3) in post-transcriptional regulation of mitochondrial gene expression.; Authors - Erik Boehm
	IF	J Biol Chem; The Pseudouridine Synthase RPUSD4 is an essential component of Mitochondrial RNA Granules.; Authors - Sofia Zaganelli
human,zebrafish	WB	Hum Mutat; Mutations in FASTKD2 are associated with mitochondrial disease with multi-OXPHOS deficiency.; Authors - Xiujuan Wei ; KD Validated
		J Cell Sci; Mitochondrial Alkbh1 localises to mtRNA granules and its knockdown induces mitochondrial UPR in humans and C. elegans.; Authors - Anita Wagner