FASTKD3 antibody (18392-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 18392-1-AP (same clone as 18392-1-PBS)
WB result of FASTKD3 antibody (18392-1-AP, 1:200) with HeLa and 293 cell lysate by Maciej Szewczyk University of Warsaw. This data was developed using the same antibody clone with 18392-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 18392-1-AP (same clone as 18392-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 18392-1-AP (FASTKD3 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 18392-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 18392-1-AP (same clone as 18392-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 18392-1-AP (FASTKD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18392-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 18392-1-AP (same clone as 18392-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18392-1-AP (FASTKD3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18392-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 18392-1-AP (same clone as 18392-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18392-1-AP (FASTKD3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18392-1-PBS in a different storage buffer formulation.
IF Staining of transfected cells using 18392-1-AP (same clone as 18392-1-PBS)
IF result of anti-FASTKD3 (18392-1-AP, 1:200) with transfected COS7 cell by Dr. Nancy Kedersha. This data was developed using the same antibody clone with 18392-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 18392-1-AP (same clone as 18392-1-PBS)
Immunofluorescent analysis of HepG2 cells using 18392-1-AP (FASTKD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18392-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 18392-1-AP (same clone as 18392-1-PBS)
Immunofluorescent analysis of HeLa cells using 18392-1-AP (FASTKD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18392-1-PBS in a different storage buffer formulation.

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

18392-1-PBS targets FASTKD3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FASTKD3 fusion protein Ag13083 Predict reactive species
Full Name	FAST kinase domains 3
Calculated Molecular Weight	76 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC001295
Gene Symbol	FASTKD3
Gene ID (NCBI)	79072
RRID	AB_2878541
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14CZ7
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

FAST kinase domain-containing protein 3(FASTKD3) belongs to FASTKD family.FASTKD3 is an essential component of mitochondrial respiration that may modulate energy balance in cells exposed to adverse conditions by functionally coupling mitochondrial protein synthesis to respiration. The expression of endogenous FASTKD3 is very weak. This antibody detects the c-terminal of FASTKD3.