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FBP1 Polyclonal antibody

FBP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12842-1-AP

Synonyms

FBP, FBP1, FBPase 1, fructose 1,6 bisphosphatase 1



Tested Applications

Positive WB detected inMCF-7 cells, human liver tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12842-1-AP targets FBP1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBP1 fusion protein Ag3837
Full Name fructose-1,6-bisphosphatase 1
Calculated molecular weight 338 aa, 37 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC012927
Gene symbol FBP1
Gene ID (NCBI) 2203
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).

Protocols

Product Specific Protocols
WB protocol for FBP1 antibody 12842-1-APDownload protocol
IHC protocol for FBP1 antibody 12842-1-APDownload protocol
IF protocol for FBP1 antibody 12842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

J Med Chem

Discovery of N-Arylsulfonyl-Indole-2-Carboxamide Derivatives as Potent, Selective, and Orally Bioavailable Fructose-1,6-Bisphosphatase Inhibitors-Design, Synthesis, In Vivo Glucose Lowering Effects, and X-ray Crystal Complex Analysis.

Authors - Jie Zhou
humanWB

Am J Cancer Res

HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma.

Authors - Li-Na Yang
humanWB

J Proteome Res

Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease.

Authors - Yong Zhou
humanIHC

Sci Rep

Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.

Authors - Katsutaka Mitachi
humanWB, IHC

Life Sci

Forced overexpression of FBP1 inhibits proliferation and metastasis in cholangiocarcinoma cells via Wnt/β-catenin pathway.

Authors - Wei Zhao
pigWB

PLoS One

Betaine supplementation in maternal diet modulates the epigenetic regulation of hepatic gluconeogenic genes in neonatal piglets.

Authors - Demin Cai