FBP1 Polyclonal antibody

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  WB analysis of MCF-7 using 12842-1-AP

  WB result of FBP1 antibody (12842-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBP1 transfected MCF-7 cells.

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  WB analysis using 12842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12842-1-AP (FBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12842-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12842-1-AP (FBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 12842-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12842-1-AP (FBP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 12842-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12842-1-AP (FBP1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 12842-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FBP1 antibody (12842-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

FBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12842-1-AP

Publications(23)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Liver FBPase, Fructose-1,6-bisphosphatase 1, FBPase 1, FBP, EC:3.1.3.11

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Tested Applications

• Positive WB detected in: MCF-7 cells, human liver tissue, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 19 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

12842-1-AP targets FBP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBP1 fusion protein Ag3837
• Full Name: fructose-1,6-bisphosphatase 1
• Calculated Molecular Weight: 338 aa, 37 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC012927
• Gene Symbol: FBP1
• Gene ID (NCBI): 2203
• RRID: AB_2103572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P09467
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).

Publications

Species	Application	Title
human,mouse	WB	J Med Chem; Discovery of N-Arylsulfonyl-Indole-2-Carboxamide Derivatives as Potent, Selective, and Orally Bioavailable Fructose-1,6-Bisphosphatase Inhibitors-Design, Synthesis, In Vivo Glucose Lowering Effects, and X-ray Crystal Complex Analysis.; Authors - Jie Zhou
human	WB	Am J Cancer Res; HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma.; Authors - Li-Na Yang
human	WB	J Proteome Res; Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease.; Authors - Yong Zhou
human	IHC	Sci Rep; Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.; Authors - Katsutaka Mitachi
human	WB, IHC	Life Sci; Forced overexpression of FBP1 inhibits proliferation and metastasis in cholangiocarcinoma cells via Wnt/β-catenin pathway.; Authors - Wei Zhao
pig	WB	PLoS One; Betaine supplementation in maternal diet modulates the epigenetic regulation of hepatic gluconeogenic genes in neonatal piglets.; Authors - Demin Cai