FBP1 Monoclonal antibody

• 

  WB analysis using 68446-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 68446-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 68446-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 68446-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 68446-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 68446-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 68446-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FBP1 antibody (68446-1-Ig, Clone: 2G4E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

FBP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 68446-1-Ig

Clone No.2G4E4

Publications(3)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FBP, FBP1, FBPase 1, fructose 1,6 bisphosphatase 1

---

Tested Applications

• Positive WB detected in: mouse liver tissue, MCF-7 cells, T-47D cells, pig liver tissue, rat liver tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:40000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 3 publications below

Product Information

68446-1-Ig targets FBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3837

  Product name: Recombinant human FBP1 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-338 aa of BC012927

  Sequence: MADQAPFDTDVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYGIAGSTNVTGDQVKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDCGVNCFMLDPAIGEFILVDKDVKIKKKGKIYSLNEGYARDFDPAVTEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLYECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ

• Full Name: fructose-1,6-bisphosphatase 1
• Calculated Molecular Weight: 338 aa, 37 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC012927
• Gene Symbol: FBP1
• Gene ID (NCBI): 2203
• RRID: AB_3085158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09467
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	J Cell Mol Med; TRIM47 Facilitates Osteosarcoma Progression via Destabilising FBP1 and Thus Activation of Wnt/β-Catenin Pathway.; Authors - Heng Wang ; KD Validated
mouse, human, bovine	WB,IF	Reprod Med Biol; The Three Pillars of ATP Production in Mammalian Sperm: Integrating Gluconeogenesis Into the Metabolic Framework.; Authors - Kosei Oishi
human	IF	Adv Sci (Weinh); TRIM47 Regulates Energy Metabolism via Glycolytic Reprogramming to Drive Hepatocellular Carcinoma Progression and Represents an Efficient Therapeutic Target.; Authors - Weijie Sun