FBP17 Polyclonal antibody

FBP17 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (2)

Cat no : 27056-1-AP

Synonyms

FBP17, FNBP1, formin binding protein 1, Formin binding protein 17, hFBP17, KIAA0554

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Validation Data Gallery

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  WB analysis of mouse brain using 27056-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27056-1-AP (FBP17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 27056-1-AP

  IP result of anti-FBP17 (IP:27056-1-AP, 4ug; Detection:27056-1-AP 1:1000) with mouse brain tissue lysate 3000ug.

• 

  IHC staining of human lung cancer using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27056-1-AP (FBP17 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27056-1-AP (FBP17 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:150-1:600
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 27056-1-AP is FBP17 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBP17 fusion protein Ag25784
• Full Name: formin binding protein 1
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC101755
• Gene Symbol: FBP17
• Gene ID (NCBI): 23048
• RRID: AB_2880734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBP17 (formin-binding protein 17) is a new dynamin-associating molecule consisting of several functional domains, including an N-terminal EFC domain and an SH3 domain at the C-terminus. FBP17 is involved in dynamin-mediated endocytosis and has been reported to be required for invadopodia formation and tumor cell invasion.

Publications

Species	Application	Title
human	WB	bioRxiv; F-BAR proteins CIP4 and FBP17 function in cortical neuron radial migration and process outgrowth; Authors - Lauren A English

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cicek (Verified Customer) (06-11-2024)

I tested the antibody on mouse neurons, iPSCs and 3-weeks-old iNeurons with Western blotting using beta-actin as positive control. There were bands at the 80 kDa mark showing FBP17 for iPSC and iNeurons, but not mouse neurons. All samples showed a strong unspecific band at 70 kDa mark.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

María (Verified Customer) (02-08-2022)

Works fine for WB (1:1000), for IF some background, even after several washes with detergent

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela/RPE-1/MEF/NaTi