FBXO44 Polyclonal antibody, PBS Only

• 

  WB analysis using 10626-1-AP (same clone as 10626-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10626-1-AP (FBXO44 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10626-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 10626-1-AP (same clone as 10626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10626-1-AP (FBXO44 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10626-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 10626-1-AP (same clone as 10626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10626-1-AP (FBXO44 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10626-1-PBS in a different storage buffer formulation.

FBXO44 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 10626-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

10626-1-PBS targets FBXO44 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0977

  Product name: Recombinant human FBXO44 protein

  Source: e coli.-derived, PKG

  Tag: GST

  Domain: 1-224 aa of BC007832

  Sequence: MAVGNINELPENILLELFTHVPARQLLLNCRLVCSLWRDLIDLVTLWKRKCLREGFITEDWDQPVADWKIFYFLRSLHRNLLHNPCAEEGFEFWSLDVNGGDEWKVEDLSRDQRKEFPNDQVRSQARLRVQVPAVRSAPVVRARASGDLPARPGDHPAEERCQVEGGLPHILQLPARRPLHLVSARRRGHSLLGRLVRPEGHQQQHHHRAPAALTPPEPPSAEP

• Full Name: F-box protein 44
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC007832
• Gene Symbol: FBXO44
• Gene ID (NCBI): 93611
• RRID: AB_2278465
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H4M3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FBXO44 is a member of the F-box protein family that shares a function as substrate recognition factors for the SKP1-CUL1-F-box (SCF)-type ubiquitin ligase. Several human FBXs (FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44) share a conserved G domain (also known as FBA domain), which is responsible for recognizing N-glycan moieties by most FBXs in this group except FBXO44. (PMID: 25970626, PMID: 23086937)