Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10626-1-AP targets FBXO44 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0977 Product name: Recombinant human FBXO44 protein Source: e coli.-derived, PKG Tag: GST Domain: 1-224 aa of BC007832 Sequence: MAVGNINELPENILLELFTHVPARQLLLNCRLVCSLWRDLIDLVTLWKRKCLREGFITEDWDQPVADWKIFYFLRSLHRNLLHNPCAEEGFEFWSLDVNGGDEWKVEDLSRDQRKEFPNDQVRSQARLRVQVPAVRSAPVVRARASGDLPARPGDHPAEERCQVEGGLPHILQLPARRPLHLVSARRRGHSLLGRLVRPEGHQQQHHHRAPAALTPPEPPSAEP Predict reactive species |
| Full Name | F-box protein 44 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC007832 |
| Gene Symbol | FBXO44 |
| Gene ID (NCBI) | 93611 |
| RRID | AB_2278465 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H4M3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FBXO44 is a member of the F-box protein family that shares a function as substrate recognition factors for the SKP1-CUL1-F-box (SCF)-type ubiquitin ligase. Several human FBXs (FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44) share a conserved G domain (also known as FBA domain), which is responsible for recognizing N-glycan moieties by most FBXs in this group except FBXO44. (PMID: 25970626, PMID: 23086937)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXO44 antibody 10626-1-AP | Download protocol |
| IHC protocol for FBXO44 antibody 10626-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FBXO44 Polyclonal antibody (catalog #10626-1-AP) has 4 total citations. These appear in Nature Communications, Journal of Controlled Release, Scientific Reports, and Journal of Ethnopharmacology. The research fields span esophagogastric junction adenocarcinoma proteomics, CRISPR/Cas9-based triple-negative breast cancer stem cell therapy, gastric cancer progression in young adults, and PXR-mediated nephrotoxicity. Cited applications include IHC and WB, with reactivity in human and mouse.
| Species | Application | Title |
|---|---|---|
Nat Commun Integrative proteomic characterization of adenocarcinoma of esophagogastric junction | ||
J Control Release Core-shell vector-mediated co-delivery of CRISPR/Cas9 system and hydrophobic drugs against triple-negative breast cancer stem cells | ||
J Ethnopharmacol Fengshi Gutong Capsules alleviate aconitums-induced nephrotoxicity via PXR-mediated efflux of aconitum alkaloid. |
Reviews
What customers say
The single review for this antibody (used in Western Blot on heart tissue at a 1:500 dilution) gave it a 3-star rating. The reviewer noted that the antibody produced some non-specific bands, but the specific Fbxo44 band at approximately 30 kDa was still clearly detectable. Overall, the feedback suggests the antibody works but may benefit from optimization to reduce background.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tongbin (Verified Customer) (08-25-2020) | This antibody had some non-specific bands in western blots. Nevertheless, the specific Fbxo44 band at around 30 kDa can still be seen.
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