FCGR2B / CD32b Polyclonal antibody

FCGR2B / CD32b Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 21541-1-AP

Synonyms

CD32, CD32B, CDw32, Fc gamma RII b, Fc gamma RIIb, FCG2, FCGR2, FCGR2B, FcRII b, IGFR2, IgG Fc receptor II b

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Validation Data Gallery

• 

  WB analysis of human placenta using 21541-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21541-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 21541-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 21541-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 21541-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 21541-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 21541-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue, Raji cells
• Positive IHC detected in: human placenta tissue, human spleen tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21541-1-AP is FCGR2B / CD32b Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FCGR2B / CD32b fusion protein Ag16189
• Full Name: Fc fragment of IgG, low affinity IIb, receptor (CD32)
• Calculated molecular weight: 310 aa, 34 kDa
• Observed molecular weight: 36 kDa
• GenBank accession number: BC031992
• Gene symbol: FCGR2B
• Gene ID (NCBI): 2213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.