Tested Applications
| Positive WB detected in | J774A.1 cells, RAW 264.7 cells, mouse spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
33531-1-AP targets FCGR4/CD16-2 in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg7179 Product name: Recombinant mouse FcgR4/CD16-2 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 21-203 aa of NM_144559.2 Sequence: GLQKAVVNLDPKWVRVLEEDSVTLRCQGTFSPEDNSIKWFHNESLIPHQDANYVIQSARVKDSGMYRCQTALSTISDPVQLEVHMGWLLLQTTKWLFQEGDPIHLRCHSWQNRPVRKVTYLQNGKGKKYFHENSELLIPKATHNDSGSYFCRGLIGHNNKSSASFRISLGDPGSPSMFPPWHQ Predict reactive species |
| Full Name | Fc receptor, IgG, low affinity IV |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | NM_144559.2 |
| Gene Symbol | Fcgr4 |
| Gene ID (NCBI) | 246256 |
| RRID | AB_3743004 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | A0A0B4J1G0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fc gamma receptors (FcγRs) are receptors for the Fc region of immunoglobulin G (IgG) antibodies and play important roles in immune responses. Mouse FCGR4, also known as CD16-2 or FcgammaRIV, is the mouse ortholog of primate FcgammaRIII (PMID: 12389094; 16039578; 17558411). FCGR4 is expressed on monocytes, macrophages, and neutrophils, and it binds to IgG2a and IgG2b with intermediate affinity (PMID: 16039578). It also acts as a receptor for the Fc region of immunoglobulin epsilon (IgE) (PMID: 17558411; 18949059).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FCGR4/CD16-2 antibody 33531-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
