FEM1A Polyclonal antibody

FEM1A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15169-1-AP

Synonyms

EPRAP, fem 1 homolog a (C. elegans), FEM1 alpha, FEM1A, Protein fem 1 homolog A

---

Validation Data Gallery

• 

  WB analysis of U-937 using 15169-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 15169-1-AP (FEM1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 15169-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15169-1-AP (FEM1A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 15169-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15169-1-AP (FEM1A antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: U-937 cells
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15169-1-AP targets FEM1A in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FEM1A fusion protein Ag7357
• Full Name: fem-1 homolog a (C. elegans)
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC004988
• Gene Symbol: FEM1A
• Gene ID (NCBI): 55527
• RRID: AB_2102618
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FEM1A, also named as Protein fem-1 homolog A-A, is a 654 amino acid protein, which Belongs to the fem-1 family. FEM1A is highly expressed in adult heart and skeletal muscle. FEM1A is a Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. FEM1A may participate in antiinflammatory signaling via its interaction with PTGER4.Furthermore, Fem1aexpression is down-regulated in rhabdomyosarcoma cells suggesting that the FEM1A gene may be involved in muscle differentiation or be at least a marker of abnormal differentiation in rhabdomyosarcoma. Knowing that a molecular weight of 76 kDa is predicted for human FEM1A, but phosphorylated FEM1A is 95-100 kDa (PMID: 19406122).