FHL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

FHL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 21619-1-AP

Synonyms

Skeletal muscle LIM protein 3, LIM domain protein DRAL, FHL-2, FHL 2, DRAL

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Validation Data Gallery

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  WB analysis using 21619-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 21619-1-AP

  IP result of anti-FHL2 (IP:21619-1-AP, 4ug; Detection:21619-1-AP 1:1000) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of rat heart using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 21619-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21619-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 21619-1-AP (FHL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 21619-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FHL2 antibody (21619-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, U2OS cells, hTERT-RPE1 cells, NIH/3T3 cells
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human cervical cancer tissue, human skin cancer tissue, mouse kidney tissue, mouse ovary tissue, rat heart tissue, rat ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 4 publications below

Product Information

21619-1-AP targets FHL2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FHL2 fusion protein Ag1378
• Full Name: four and a half LIM domains 2
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC014397
• Gene Symbol: FHL2
• Gene ID (NCBI): 2274
• RRID: AB_10860263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.; Authors - Xiaojie Xu
human	WB,IF,CoIP	EBioMedicine; Up-regulated FHL2 inhibits ovulation through interacting with androgen receptor and ERK1/2 in polycystic ovary syndrome.; Authors - Ruiqiong Zhou
human,mouse	WB,IHC,IF,CoIP	Cell Death Dis; FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells; Authors - Chen Wang ; KD Validated
mouse	CoIP,WB	iScience; Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice; Authors - Lu Ren
human	WB	Int J Mol Sci; FHL2 Inhibits SARS-CoV-2 Replication by Enhancing IFN-β Expression through Regulating IRF-3; Authors - Zhiqiang Xu ; KD Validated
human	WB, IHC	J Virol; Structural and Functional Characterization of Host FHL1 Protein Interaction with Hypervariable Domain of Chikungunya Virus nsP3 Protein.; Authors - Tetyana Lukash