FHOD3 Polyclonal antibody

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  IHC staining of mouse heart using 31744-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31744-1-AP (FHOD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 31744-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31744-1-AP (FHOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 31744-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using FHOD3 antibody (31744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 31744-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using FHOD3 antibody (31744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FHOD3 Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 31744-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Synonyms

CMH28, FHOS2, hFHOS2, KIAA1695

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Tested Applications

• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31744-1-AP targets FHOD3 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36247

  Product name: Recombinant human FHOD3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 650-825 aa of BC157093

  Sequence: RTTGLWPAGVQDAGVNGQCGDILTNKRFMLDMLYAHNRKSPDDEEKGDGEAGRTQQEAEAVASLATRISTLQANSQTQDESVRRVDVGCLDNRGSVKAFAEKFNSGDLGRGSISPDAEPNDKVPETAPVQPKTESDYIWDQLMANPRELRIQDMDFTDLGEEDDIDVLDVDLGHRE

• Full Name: formin homology 2 domain containing 3
• GenBank Accession Number: BC157093
• Gene Symbol: FHOD3
• Gene ID (NCBI): 80206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q2V2M9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FHOD3, also known as FHOS2 or Formactin2, was an important member of Formin homology proteins. FHOD3 plays a crucial role in organizing the cytoskeleton, primarily by regulating actin filament polymerization within cardiomyocytes, which is essential for heart muscle structure and function. Mutations in this gene are associated with familial cardiomyopathies, including dilated cardiomyopathy (DCM), and its protein levels are altered in hypertrophic cardiomyopathy (HCM). FHOD3 is expressed abundantly in the heart (PMID: 32447646, PMID: 33251537).