FKBP2 Polyclonal antibody

FKBP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11700-1-AP

Synonyms

13 kDa FK506 binding protein, 13 kDa FKBP, FK506 binding protein 2, FK506 binding protein 2, 13kDa, FKBP 13, FKBP 2, FKBP13, FKBP2, Immunophilin FKBP13, PPIase, PPIase FKBP2, Rotamase

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Validation Data Gallery

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  WB analysis of mouse cerebellum using 11700-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 11700-1-AP (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11700-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11700-1-AP (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11700-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11700-1-AP (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 11700-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11700-1-AP (FKBP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11700-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11700-1-AP (FKBP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, HeLa cells, MCF-7 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

11700-1-AP targets FKBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FKBP2 fusion protein Ag2255
• Full Name: FK506 binding protein 2, 13kDa
• Calculated Molecular Weight: 142 aa, 16 kDa
• Observed Molecular Weight: 13-16 kDa
• GenBank Accession Number: BC003384
• Gene Symbol: FKBP2
• Gene ID (NCBI): 2286
• RRID: AB_2102876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBP2 is also named as FKBP13 and belongs to the FKBP-type PPIase family. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP2 has a 21-amino acid signal peptide and appears to be membrane-associated(PMID:1713687). It is localized to the lumen of the endoplasmic reticulum (ER). FKBP12 and FKBP13 are highly similar proteins, of molecular masses 12 kDa and 13 kDa respectively, with approx.43 % amino acid identity. The strong homology between FKBP12 and FKBP13 suggests that they may share similar biological functions, although, apart from rotamase activity, details relating to the function of either protein are scant(PMID:8373365).

Publications

Species	Application	Title
human	WB	J Cell Physiol; Multiple roles for peptidylglycine α-amidating monooxygenase in the response to hypoxia.; Authors - Vishwanatha K S Rao
mouse	WB	Endocrinology; Changes in Corticotrope Gene Expression Upon Increased Expression of Peptidylglycine α-Amidating Monooxygenase.; Authors - Richard E Mains