FMNL1 Monoclonal antibody, PBS Only

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  WB analysis using 67587-1-Ig (same clone as 67587-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67587-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67587-1-Ig (same clone as 67587-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67587-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67587-1-Ig (same clone as 67587-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67587-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NK-92 using 67587-1-Ig (same clone as 67587-1-PBS)

  NK-92 cells were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67587-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67587-1-Ig (same clone as 67587-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using FMNL1 antibody (67587-1-Ig, Clone: 1B12A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67587-1-PBS in a different storage buffer formulation.

FMNL1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67587-1-PBS

Clone No.1B12A4

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

1B12A4, CLL-associated antigen KW-13, FMNL, Formin-like protein 1

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Product Information

67587-1-PBS targets FMNL1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27322

  Product name: Recombinant human FMNL1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 357-501 aa of BC111564

  Sequence: LYLERLRLTESDKLQVQIQAYLDNIFDVGALLEDTETKNAVLEHMEELQEQVALLTERLRDAENESMAKIAELEKQLSQARKELETLRERFSESTAMGPSRRPPEPEKAPPAAPTRPSALELKVEELEEKGLIRILRGPGDAVSI

• Full Name: formin-like 1
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC111564
• Gene Symbol: FMNL1
• Gene ID (NCBI): 752
• RRID: AB_2882796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95466
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. 67587-1-Ig antibody detects the protein around 140-150 kDa which is similar to the results reported in publications.(PMID: 21868368, 22986496 , 23182705)