FMO3 Polyclonal antibody

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• KD/KO Validated

FMO3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17469-1-AP

Synonyms

Dimethylaniline oxidase 3, FMO 3, FMO form 2, FMO II, FMO3, FMOII, TMAU

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Validation Data Gallery

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  WB analysis of mouse liver using 17469-1-AP

  WB results of FMO3 antibody (17469-1-AP) with 8-week old CD1 male and female mice. (FMO3 is not expressed in male mice after 6-weeks of age and therefore are natural knockouts.).

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  WB analysis using 17469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17469-1-AP (FMO3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 17469-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17469-1-AP (FMO3 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 17469-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17469-1-AP (FMO3 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:100-1:500
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

17469-1-AP targets FMO3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FMO3 fusion protein Ag11405
• Full Name: flavin containing monooxygenase 3
• Calculated Molecular Weight: 532 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC032016
• Gene Symbol: FMO3
• Gene ID (NCBI): 2328
• RRID: AB_2878410
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Microsomal flavin-containing monooxygenase (FMO) catalyzes the FAD-, NADPH-, and O2-dependent oxidation of a large number of xenobiotics containing soft nucleophiles,including alkaloids, pesticides, and pharmaceutical substances.Based on the cDNA sequence, the FMOs are classified into five subfamilies (FMO1 to FMO5)(PMID:11792679).In human beings ,FMO3 is predominant in the adult liver,but not appear sex-dependent in the tissue.In the mouse liver,its expression has been shown to be sex-dependent and expressed only in females(PMID:11996886).Defects in FMO3 are the cause of trimethylaminuria (TMAU).This antibody is specific to FMO3.

Publications

Species	Application	Title
mouse	WB	J Hazard Mater; Dietary titanium dioxide particles (E171) promote diet-induced atherosclerosis through reprogramming gut microbiota-mediated choline metabolism in APOE-/- mice.; Authors - Xiaoqiang Zhu
rat	WB	EBioMedicine; Akkermansia muciniphila prevents cold-related atrial fibrillation in rats by modulation of TMAO induced cardiac pyroptosis.; Authors - Yingchun Luo
mouse	WB	Front Microbiol; Berberine Improves Vascular Dysfunction by Inhibiting Trimethylamine-N-oxide via Regulating the Gut Microbiota in Angiotensin II-Induced Hypertensive Mice.; Authors - Zhichao Wang
rat	WB	Food Sci Biotechnol; Effects of black raspberry extract on gut microbiota, microbial metabolites, and expressions of the genes involved in cholesterol and bile acid metabolisms in rats fed excessive choline with a high-fat diet; Authors - Taehwan Lim