PSMA/GCPII Polyclonal antibody

PSMA/GCPII Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 13163-1-AP

Synonyms

FGCP, Folate hydrolase 1, FOLH, FOLH1, GCP2, GCPII, Glutamate carboxypeptidase 2, Glutamate carboxypeptidase II, mGCP, NAALAD1, NAALAdase, NAALADase I, PSM, PSMA, PSMA/GCPII

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Validation Data Gallery

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  WB analysis using 13163-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 13163-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 13163-1-AP (PSMA/GCPII antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13163-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13163-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13163-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human gliomas using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human gliomas using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13163-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of LNCaP using 13163-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using 13163-1-AP (PSMA/GCPII antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, mouse kidney tissue, human liver tissue, rat kidney tissue, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue, human gliomas tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 3 publications below
• FC: See 1 publications below

Product Information

13163-1-AP targets PSMA/GCPII in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMA/GCPII fusion protein Ag3812
• Full Name: folate hydrolase (prostate-specific membrane antigen) 1
• Calculated Molecular Weight: 719 aa, 81 kDa
• Observed Molecular Weight: 100 kDa, 120 kDa
• GenBank Accession Number: BC025672
• Gene Symbol: FOLH1
• Gene ID (NCBI): 2346
• RRID: AB_2106442
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,FC	ACS Sens; Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins.; Authors - Xiaoyi Gao
	IHC	Eur J Nucl Med Mol Imaging; Prostate Specifc Membrane Antigen in Human and Mouse Tissue; Authors -
mouse	IHC	J Nucl Med; Molecular imaging of Neuroendocrine Prostate Cancer by targeting Delta-like Ligand 3.; Authors - Joshua Aaron Korsen
human	WB	J Control Release; PSMA-targeted nanoparticles for specific penetration of blood-brain tumor barrier and combined therapy of brain metastases.; Authors - Jiang Ni
rat	WB	Front Pharmacol; Increasing Aspartoacylase in the Central Amygdala: The Common Mechanism of Gastroprotective Effects of Monoamine-Based Antidepressants Against Stress.; Authors - Kaiyun Yao
human	IHC	Appl Radiat Isot; Autoradiography on deparaffinized tissue sections - A feasibility study with 68Ga-labeled PET-tracers; Authors - Eva-Maria Klebermass