PSMA/GCPII Recombinant antibody

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  WB analysis of LNCaP using 84615-2-RR

  LNCaP cells were subjected to SDS PAGE followed by western blot with 84615-2-RR (FOLH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of LNCaP using 84615-2-RR

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using FOLH1 antibody (84615-2-RR, Clone: 242026F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84615-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84615-2-RR against Human PSMA/GCPII were performed. The affinity constant is 1.26 nM.

PSMA/GCPII Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84615-2-RR

Clone No.242026F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

FOLH1, PSMA, 242026F4, Cell growth-inhibiting gene 27 protein, EC:3.4.17.21

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Tested Applications

• Positive WB detected in: LNCaP cells
• Positive IF/ICC detected in: LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84615-2-RR targets PSMA/GCPII in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PSMA/GCPII fusion protein Eg0216
• Full Name: folate hydrolase (prostate-specific membrane antigen) 1
• Calculated Molecular Weight: 84KD
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_004476.3
• Gene Symbol: PSMA
• Gene ID (NCBI): 2346
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q04609
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Prostate-specific membrane antigen (PSMA), also known as glutamate carboxypeptidase II (GCPII), is a type II transmembrane glycoprotein that has gained significant attention in the field of prostate cancer (PCa) diagnostics and therapeutics. PSMA is highly overexpressed in prostate cancer tissue, especially in aggressive, androgen-deprived, metastatic, and hormone-refractory prostate cancers. Up to 95% of prostate adenocarcinomas express PSMA receptors on their surface. Physiological expression of PSMA is observed in normal prostate epithelium, duodenum, colon, renal tubules, and sympathetic ganglia. It is also found in other tissues such as the salivary glands, lacrimal glands, and various parts of the gastrointestinal tract, though at lower levels.